Molecular forms of atrial natriuretic peptides in dog atrium and plasma

The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all...

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Bibliographic Details
Published inLife sciences (1973) Vol. 42; no. 24; p. 2533
Main Authors Cernacek, P, Maher, E, Crawhall, J C, Levy, M
Format Journal Article
LanguageEnglish
Published Netherlands 1988
Subjects
Animals
Atrial Natriuretic Factor - analysis
Atrial Natriuretic Factor - blood
Chromatography, High Pressure Liquid
Dogs
Heart Atria - analysis
Humans
Macromolecular Substances
Molecular Weight
Myocardium - analysis
Protein Precursors - analysis
Radioimmunoassay
Tissue Distribution
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Summary:The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.
ISSN:0024-3205
DOI:10.1016/0024-3205(88)90353-0