Characteristics of mannanase enzyme in actinomycetes isolated from Taman Nasional Bukit Duabelas Jambi

Indonesia has about 14.03 million hectars of palm oil plantation. Palm kernel cake (PKC) is a byproduct of palm oil processing majority consist of β-mannan. Mannan is a component of hemicellulose which have an economic value. Mannan can be converted to the manooligosaccharides (MOS) using bacterial...

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Bibliographic Details
Published inIOP conference series. Earth and environmental science Vol. 457; no. 1; pp. 12070 - 12078
Main Authors Evan, S, Meryandini, A, Sunarti, T C
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.02.2020
Subjects
Actinomycetes
Enzymatic activity
Enzyme activity
Enzymes
Hemicellulose
Mannan
Mannanases
mannanolytic
mannooligosaccharides
palm kernel cake
Palm oil
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Summary:Indonesia has about 14.03 million hectars of palm oil plantation. Palm kernel cake (PKC) is a byproduct of palm oil processing majority consist of β-mannan. Mannan is a component of hemicellulose which have an economic value. Mannan can be converted to the manooligosaccharides (MOS) using bacterial enzyme. This study aimed to produce MOS from PKC using actinomycetes from Taman Nasional Bukit Duabelas Jambi. The selection was begun with the production of the clear zone on medium containing PKC then enzyme activity was measured. Isolate HJ4-5B had the highest mannanolytic index up to 0.4. The enzyme activity of isolate HJ4-5B increased significantly up to 0.895 U/mL at 7th day of culture incubation. The highest mannanase activity of isolate HJ4-5B occurs at pH 6 and 70 °C with an incubation time of 7th day which reached 1.186 U/mL.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/457/1/012070