Development and characterization of a codominant marker linked to root-knot nematode resistance, and its application to peach rootstock breeding

• Published in: Theoretical and applied genetics Vol. 99; no. 1/2; pp. 115 - 122
• Main Authors: Lu, Z.X, Sossey-Alaoui, K, Reighard, G.L, Baird, W.V, Abbott, A.G
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.07.1999; Berlin Springer Nature B.V
• Subjects: alleles; amplified fragment length polymorphism; Biological and medical sciences; Classical genetics, quantitative genetics, hybrids; codominance; Crop diseases; Crops; crossing; Cultivars; Fundamental and applied biological sciences. Psychology; Genes; genetic markers; Genetics of eukaryotes. Biological and molecular evolution; Genomes; hybrids; Meloidogyne incognita; Meloidogyne javanica; molecular sequence data; Nematoda; Nematodes; nucleotide sequences; pest resistance; Polymorphism; Prunus; Prunus persica; Pteridophyta, spermatophyta; rootstocks; segregation; sequence tagged sites; Trees; Vegetals; United States > US; Japan; Okinawa; Genetic mapping; Linkage; Genetic marker; Rosaceae; Chromosome; Prunus persica; Germplasm; Pest; Meloidogyne incognita; Dicotyledones; Angiospermae; Genetic improvement; Helmintha; Pest resistance; Nemathelminthia; Spermatophyta; Fruit tree; Invertebrata; Nematoda; Meloidogyne javanica
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220051215

The presence of a codominant AFLP marker, EAA/MCAT10, correlates with the primary source of resistance to root-knot nematodes (Meloidogyne incognita and M. javanica) in rootstock cultivars of peach [Prunus persica (L.) Batsch]. Two allelic DNA fragments of this AFLP marker were cloned, sequenced and converted to sequence tagged sites (STS). Four nucleotide differences (i.e. one addition and three substitutions) were observed between the two clones. Furthermore, there was a diagnostic Sau3 AI cleavage site (GATC) in the large fragment that was absent from the small fragment (GTTC at this site). The applicability of this STS marker system to peach germplasm improvement was evaluated: genomic DNAs of cross parents (i.e. 'Lovell' and 'Nemared'), four F(1) hybrids (K62-67, K62-68, P101-40 and P101-41) and two F(2) populations (from K62-68 and P101-41), as well as DNA from a test panel of 18 rootstock cultivars or selections, were PCR-amplified with the Mij3F/Mij1R primer pair and then digested with Sau3 AI. The banding patterns showed that the EAA/MCAT10 STS markers can clearly distinguish the three genotypes--homozygous resistant, heterozygous resistant and homozygous susceptible--in the 'Lovell' x 'Nemared' cross. In addition, results from the rootstock survey were consistent with each rootstock's phenotypic response to nematode infection, except for 'Okinawa', 'Flordaguard' and 'Yunnan' where root-knot resistance may have arisen independently. Therefore, the EAA/MCAT10 STS markers will be a useful tool to initiate marker assisted selection studies in peach rootstock breeding for root-knot nematode resistance.