Disposable Non-Enzymatic Glucose Sensors Using Screen-Printed Nickel/Carbon Composites on Indium Tin Oxide Electrodes

• Published in: Sensors (Basel, Switzerland) Vol. 15; no. 12; pp. 31083 - 31091
• Main Authors: Jeon, Won-Yong, Choi, Young-Bong, Kim, Hyug-Han
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 10.12.2015; MDPI AG
• Subjects: Biosensing Techniques - instrumentation; Biosensing Techniques - methods; biosensor; Carbon - chemistry; disposable glucose sensor; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrodes; Glucose - analysis; Nickel - chemistry; nickel/carbon composite; non-enzymatic glucose sensor; Surface Properties; Tin Compounds - chemistry; disposable glucose sensor; biosensor; nickel/carbon composite; non-enzymatic glucose sensor
• ISSN: 1424-8220; 1424-8220
• DOI: 10.3390/s151229846

Disposable screen-printed nickel/carbon composites on indium tin oxide (ITO) electrodes (DSPNCE) were developed for the detection of glucose without enzymes. The DSPNCE were prepared by screen-printing the ITO substrate with a 50 wt% nickel/carbon composite, followed by curing at 400 °C for 30 min. The redox couple of Ni(OH)₂/NiOOH was deposited on the surface of the electrodes via cyclic voltammetry (CV), scanning from 0-1.5 V for 30 cycles in 0.1 M NaOH solution. The DSPNCE were characterized by field-emission scanning electron microscopy (FE-SEM), X-ray photoelectron spectroscopy (XPS), and electrochemical methods. The resulting electrical currents, measured by CV and chronoamperometry at 0.65 V vs. Ag/AgCl, showed a good linear response with glucose concentrations from 1.0-10 mM. Also, the prepared electrodes showed no interference from common physiologic interferents such as uric acid (UA) or ascorbic acid (AA). Therefore, this approach allowed the development of a simple, disposable glucose biosensor.