Identification of Endophytic and Rhizosphere Bacteria in Maize (Zea mays L.) in Limapuluh Kota Region, West Sumatra, Indonesia

The variety of microorganisms that are very abundant began to be used for the benefit of sustainable agriculture. A group of non-pathogenic bacteria has been reported to control disease-causing pathogens and improve plant growth. The purpose of this study is (a) to identify and characterize endophyt...

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Bibliographic Details
Published inIOP conference series. Earth and environmental science Vol. 347; no. 1; pp. 12002 - 12009
Main Authors Sondang, Y, Anty, K, Siregar, R
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.11.2019
Subjects
Bacteria
Body density
Colonies
Color
Corn
Endophytes
Microorganisms
Plant growth
Random sampling
Rhizosphere
Roots
rRNA 16S
Sampling
Sampling methods
Soil bacteria
Soil microorganisms
Statistical sampling
Stimulants
Surface roughness
Sustainable agriculture
West Sumatra Indonesia
Sumatra
Indonesia
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Summary:The variety of microorganisms that are very abundant began to be used for the benefit of sustainable agriculture. A group of non-pathogenic bacteria has been reported to control disease-causing pathogens and improve plant growth. The purpose of this study is (a) to identify and characterize endophytic bacteria and corn rhizosphere (b) to study endophytic bacteria and rhizosphere which have the potential to spur plant growth. The experiment was carried out at the Payakumbuh State of Agricultural Polytechnic Laboratory, Limapuluh Kota Region, West Sumatra, and LIPI Bogor. The sampling method was carried out by random sampling at four locations. Endophytic bacteria taken from the roots of maize and rhizosphere bacteria originated from the soil around the roots. Bacterial isolation was carried out by the pour and the streak plate method. Identification of bacterial characteristics based on the form of colonies, edges, surfaces, rough surface roughness, surface color, pigment color, bacterial body density, and halo zone colonies. Six species of bacteria have been identified using molecular analysis based on 16S rRNA gene fragments. Bacteria are dominated by the Bacillus genera compared to the Pseudomonas genera. The ability of phosphate dissolving bacteria indicates that endophytic bacteria and the rhizosphere act as growth stimulants.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/347/1/012002