Recognition of prokaryotic transcription terminators by spinach chloroplast RNA polymerase

To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro, we have fused the spinach chloroplast rbcL promoter to the 3' end of the rbcL gene as well as to various factor independent transcription terminators from E. coli. Transcription of the rbcL minigen...

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Bibliographic Details
Published inNucleic acids research Vol. 16; no. 17; pp. 8411 - 8431
Main Authors CHEN, L. J, OROZCO, E. M. JR
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 12.09.1988
Subjects
Base Sequence
Biological and medical sciences
Chloroplasts - enzymology
DNA Restriction Enzymes
DNA-Directed RNA Polymerases - metabolism
Escherichia coli
Fundamental and applied biological sciences. Psychology
Genes
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Plants - enzymology
Plants - genetics
Promoter Regions, Genetic
Ribulose-Bisphosphate Carboxylase - genetics
Spinacea oleracea
Spinacia oleracea
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
In vitro transcription
Gel electrophoresis
Enzyme
Escherichia coli
RNA polymerase
Spinacia oleracea
Chenopodiaceae
Plasmid
Promoter
Transcription termination
Vegetable crop
Dicotyledones
Angiospermae
Bacteria
Chloroplast RNA
Spermatophyta
Spinach
Transcription factor
Escherichieae
Nucleic acid
Chloroplast
Comparative study
Enterobacteriaceae
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Summary:To determine whether chloroplast RNA polymerase will accurately terminate transcription in vitro, we have fused the spinach chloroplast rbcL promoter to the 3' end of the rbcL gene as well as to various factor independent transcription terminators from E. coli. Transcription of the rbcL minigene did not result in production of the expected 265 nucleotide RNA. However, the spinach chloroplast RNA polymerase did terminate transcription with varying efficiency at the thra, rrnB, rrnC and gene 32 terminators. The most efficient transcription termination was observed for the threonine attenuator. For each of the prokaryotic terminators, the chloroplast enzyme ceased transcription at essentially the same position as the E. coli RNA polymerase. These data indicate that the transcription termination process in chloroplasts has some features in common with the mechanism used in prokaryotes.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/16.17.8411