Pericyte-specific deletion of Ninjurin-1 induces fragile vasa vasorum formation and enhances intimal hyperplasia of injured vasculature

• Published in: American journal of physiology. Heart and circulatory physiology Vol. 320; no. 6; pp. H2438 - H2447
• Main Authors: Horiuchi, Kiwamu, Kano, Kohei, Minoshima, Akiho, Hayasaka, Taiki, Yamauchi, Atsushi, Tatsukawa, Takamitsu, Matsuo, Risa, Yoshida, Yuri, Tomita, Yui, Kabara, Maki, Nakagawa, Naoki, Takehara, Naofumi, Hasebe, Naoyuki, Kawabe, Jun-Ichi
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.06.2021
• Subjects: Abnormalities; Angiogenesis; Arteries; Arteriosclerosis; Atherosclerosis; Blood vessels; Cytokines; Decoloring; Deletion; Dextran; Dextrans; Extravasation; Femoral artery; Fluorescein; Fluorescein isothiocyanate; Fragility; Hyperplasia; Inflammation; Injury prevention; Insertion; Macrophages; Maturation; Pericytes; Stents; vascular injury; intimal hyperplasia; vasa vasorum; angiogenesis; pericytes
• ISSN: 0363-6135; 1522-1539
• DOI: 10.1152/ajpheart.00931.2020

Adventitial abnormalities including enhanced vasa vasorum malformation are associated with development and vulnerability of atherosclerotic plaque. However, the mechanisms of vasa vasorum malformation and its role in vascular remodeling have not been fully clarified. We recently reported that Ninjurin-1 (Ninj1) is a crucial adhesion molecule for pericytes to form matured neovessels. The purpose is to examine if Ninj1 regulate adventitial angiogenesis and affects the vascular remodeling of injured vessels using pericyte-specific Ninj1 deletion mouse model. Mouse femoral arteries were injured by insertion of coiled wire. Four weeks after vascular injury, fixed arteries were decolorized. Vascular remodeling, including intimal hyperplasia and adventitial microvessel formation were estimated in three-dimensional view. Vascular fragility, including blood leakiness was estimated by extravasation of FITC-lectin or -dextran from microvessels. Ninj1 expression was increased in pericytes in response to vascular injury. NG2-CreER/Ninj1 mice were treated with tamoxifen (Tam) to induce deletion of Ninj1 in pericyte (Ninj1KO). Tam-treated-NG2-CreER or Tam-nontreated NG2-CreER/Ninj1 mice were used as controls. Intimal hyperplasia was significantly enhanced in Ninj1KO compared with controls. Vascular leakiness was significantly enhanced in Ninj1KO. In Ninj1KO, the number of infiltrated macrophages in adventitia was increased, along with the expression of inflammatory cytokines. In conclusion, deletion of Ninj1 in pericytes induces the immature vasa vasorum formation of injured vasculature and exacerbates adventitial inflammation and intimal hyperplasia. Thus, Ninj1 contributes to the vasa vasorum maturation in response to vascular injury, and to reduction of vascular remodeling.