Real-time In vivo Diagnosis of Nasopharyngeal Carcinoma Using Rapid Fiber-Optic Raman Spectroscopy

• Published in: Theranostics Vol. 7; no. 14; pp. 3517 - 3526
• Main Authors: Lin, Kan, Zheng, Wei, Lim, Chwee Ming, Huang, Zhiwei
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher 01.01.2017
• Subjects: Carcinoma - chemistry; Carcinoma - diagnostic imaging; Carcinoma - pathology; Endoscopy - methods; Female; Fiber Optic Technology - methods; Humans; Male; Middle Aged; Nasopharyngeal Neoplasms - chemistry; Nasopharyngeal Neoplasms - diagnostic imaging; Nasopharyngeal Neoplasms - pathology; Principal Component Analysis - methods; Research Paper; Sensitivity and Specificity; Spectrum Analysis, Raman - methods; Nasopharyngeal carcinoma; Fiber-optic Raman spectroscopy; In vivo diagnosis
• ISSN: 1838-7640; 1838-7640
• DOI: 10.7150/thno.16359

We report the utility of a simultaneous fingerprint (FP) (i.e., 800-1800 cm ) and high-wavenumber (HW) (i.e., 2800-3600 cm ) fiber-optic Raman spectroscopy developed for real-time diagnosis of nasopharyngeal carcinoma (NPC) at endoscopy. A total of 3731 high-quality FP/HW Raman spectra (normal=1765; cancer=1966) were acquired in real-time from 204 tissue sites (normal=95; cancer=109) of 95 subjects (normal=57; cancer=38) undergoing endoscopic examination. FP/HW Raman spectra differ significantly between normal and cancerous nasopharyngeal tissues that could be attributed to changes of proteins, lipids, nucleic acids, and the bound water content in NPC. Principal components analysis (PCA) and linear discriminant analysis (LDA) together with leave-one subject-out, cross-validation (LOO-CV) were implemented to develop robust Raman diagnostic models. The simultaneous FP/HW Raman spectroscopy technique together with PCA-LDA and LOO-CV modeling provides a diagnostic accuracy of 93.1% (sensitivity of 93.6%; specificity of 92.6%) for nasopharyngeal cancer identification, which is superior to using either FP (accuracy of 89.2%; sensitivity of 89.9%; specificity of 88.4%) or HW (accuracy of 89.7%; sensitivity of 89.0%; specificity of 90.5%) Raman technique alone. Further receiver operating characteristic (ROC) analysis reconfirms the best performance of the simultaneous FP/HW Raman technique for diagnosis of NPC. This work demonstrates for the first time that simultaneous FP/HW fiber-optic Raman spectroscopy technique has great promise for enhancing real-time cancer diagnosis in the nasopharynx during endoscopic examination.