Anti-apoptotic proteins, apoptotic and proliferative parameters and their prognostic significance in cervical carcinoma

• Published in: European journal of cancer (1990) Vol. 37; no. 9; pp. 1104 - 1110
• Main Authors: Liu, S.S, Tsang, B.K, Cheung, A.N.Y, Xue, W.C, Cheng, D.K.L, Ng, T.Y, Wong, L.C, Ngan, H.Y.S
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.2001; Elsevier
• Subjects: Adult; Aged; Aged, 80 and over; Apoptosis - physiology; Apoptotic (AI) and mitotic indices (MI); Biological and medical sciences; Carcinoma, Squamous Cell - pathology; Cell Division; Cervical carcinoma and prognosis; Female; Female genital diseases; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemical staining; Immunohistochemistry; In Situ Nick-End Labeling - methods; Inhibitor of apoptotic protein (IAP); Ki-67 Antigen - analysis; Ki-67 index; Medical sciences; Middle Aged; Mitosis - physiology; Prognosis; Retrospective Studies; Survival Analysis; Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuracil triphosphate (dUTP) nick-end labelling (TUNEL); Tumors; Uterine Cervical Neoplasms - pathology; Cervical carcinoma and prognosis; Ki-67 index; Inhibitor of apoptotic protein (IAP); Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuracil triphosphate (dUTP) nick-end labelling (TUNEL); Immunohistochemical staining; Apoptotic (AI) and mitotic indices (MI); Human; Immunohistochemistry; Cell proliferation; Correlation; Carcinoma; Prognosis; Stage classification; Tumoral marker; Uterine cervix; Malignant tumor; Gene expression; Female genital diseases; Female; Uterine cervix diseases; Apoptosis
• ISSN: 0959-8049; 1879-0852
• DOI: 10.1016/S0959-8049(01)00085-5

The inhibitor of apoptosis proteins (IAP) suppress apoptosis induced by a variety of stimuli. The aims of this study were to: (a) compare the expression of X-linked IAP (Xiap) and Human IAP-2 (Hiap-2) in cervical carcinoma cells and normal cervix, (b) determine the correlation between IAP expression and tumour apoptosis or proliferation, and (c) assess their prognostic significance in cervical carcinomas. Paraffin-embedded tissue sections were retrieved from 77 patients with cervical squamous carcinomas prior to treatments and 47 normal subjects. Tumour apoptosis was determined by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuracil triphosphate (dUTP) nick-end labelling (TUNEL) and apoptotic index (AI), and the proliferative rate was measured by Ki-67 and mitotic (MI) indices. Immunoreactive Xiap and Hiap-2 were found in both cervical cancer cells and normal tissues. IAP expressions in cancers did not correlate with apoptotic and proliferative parameters, disease stage and patient survival. The lower AI and Ki-67 index were associated with a better survival. In conclusion, the basal expression levels of IAPs have no prognostic significance, but AI and Ki-67 expression are potential prognostic indicators in cervical carcinoma.