Retinoic acid-induced alveolar cellular growth does not improve function after right pneumonectomy

• Published in: Journal of applied physiology (1985) Vol. 96; no. 3; pp. 1090 - 1096
• Main Authors: Dane, D. Merrill, Yan, Xiao, Tamhane, Rahul M, Johnson, Robert L., Jr, Estrera, Aaron S, Hogg, Deborah C, Hogg, Richard T, Hsia, Connie C. W
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01.03.2004; American Physiological Society
• Subjects: Animals; Biological and medical sciences; Cell Division - drug effects; Cell Division - physiology; Dogs; Fundamental and applied biological sciences. Psychology; Lung Volume Measurements - methods; Lungs; Male; Pneumonectomy - methods; Pneumonectomy - statistics & numerical data; Pulmonary Alveoli - cytology; Pulmonary Alveoli - drug effects; Pulmonary Alveoli - growth & development; Pulmonary Diffusing Capacity - drug effects; Pulmonary Diffusing Capacity - physiology; Respiratory system; Tomography; Tretinoin - pharmacology; Fissipedia; Carnivora; Growth; Lung; Retinoid; compensatory lung growth; Respiratory system; carbon monoxide diffusing capacity; Vertebrata; Mammalia; computerized tomographic scan; Nitric oxide; nitric oxide diffusing capacity; Diffusing capacity; Carbon monoxide; Dog; Retinoic acid
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/japplphysiol.00900.2002

Departments of 1 Internal Medicine and 2 Cardiovascular and Thoracic Surgery, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9034 Submitted 30 September 2002 ; accepted in final form 15 August 2003 To determine whether all- trans retinoic acid (RA) treatment enhances lung function during compensatory lung growth in fully mature animals, adult male dogs ( n = 4) received 2 mg·kg -1 ·day -1 po RA 4 days/wk beginning the day after right pneumonectomy (R-PNX, 55-58% resection). Litter-matched male R-PNX controls ( n = 4) received placebo. After 3 mo, transpulmonary pressure (TPP)-lung volume relationship, diffusing capacities for carbon monoxide and nitric oxide, cardiac output, and septal volume (V tiss-RB ) were measured under anesthesia by a rebreathing technique at two lung volumes. Lung air and tissue volumes (V air-CT and V tiss-CT ) were also measured from high-resolution computerized tomographic (CT) scans at a constant TPP. In RA-treated dogs compared with controls, TPP-lung volume relationships were similar. Diffusing capacities for carbon monoxide and nitric oxide were significantly impaired at a lower lung volume but similar at a high lung volume. Whereas V tiss-RB was significantly lower at both lung volumes in RA-treated animals, V air-CT and V tiss-CT were not different between groups; results suggest uneven distribution of ventilation consistent with distortion of alveolar geometry and/or altered small airway function induced by RA. We conclude that RA does not improve resting pulmonary function during the early months after R-PNX despite histological evidence of its action in enhancing alveolar cellular growth in the remaining lung. compensatory lung growth; dog; carbon monoxide diffusing capacity; nitric oxide diffusing capacity; computerized tomographic scan Address for reprint requests and other correspondence: C. C. W. Hsia, Pulmonary and Critical Care Medicine, Dept. of Internal Medicine, Univ. of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX 75390-9034.