High Concentration of Benzyladenine Solution Stimulates Anthers for Inducing Callus in Ricinus Communis L

• Published in: IOP conference series. Materials Science and Engineering Vol. 275; no. 1; pp. 12011 - 12016
• Main Authors: Liu, Ying, Yan, Shuying, Yang, Fuguang, Li, Dongliang, Tang, Jianian, Liu, Guoxuan, Lin, Shiwan, Niu, Sufang, Yang, Yali
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.12.2017
• ISSN: 1757-8981; 1757-899X
• DOI: 10.1088/1757-899X/275/1/012011

An high-frequency protocol for induction of callus from anther explants of Ricinus communis was described. When anther explants of R. communis was cultured directly onto medium containing 6-benzylaminopurine (BA) induced formation of only poor quality callus that had a low induction frequency of anther callus (10.67%). However, treating the anther explants with high concentrations (7.5-120 mg/L) of BA solution for short time periods (5-80 min) helped to improve the induction frequency and enhance the quality of the callus formation significantly. The best callus induction (41.25%) was observed when anther explants were treated with 15 mg/L BA solution for 10 min before being inoculated onto hormone-free Murashige and Skoog (MS) medium for 30 days. In order to further optimize the culture system, after treated with 15 mg/L BA for 10 min, anther explants were inoculated on the hormone-free MS medium contained concentrations of sodium nitroprusside (SNP). The results showed that SNP significantly promoted the response of callus induction, especially when 8 mg/L SNP was applied, the the highest percentage of callus induction (60.37%) were gained.