Marine-Derived Penicillium purpurogenum Reduces Tumor Size and Ameliorates Inflammation in an Erlich Mice Model

• Published in: Marine drugs Vol. 18; no. 11; p. 541
• Main Authors: Teles, Amanda Mara, Pontes, Leticia Prince Pereira, Guimarães, Sulayne Janayna Araújo, Butarelli, Ana Luiza, Silva, Gabriel Xavier, do Nascimento, Flavia Raquel Fernandes, de Barros Bezerra, Geusa Felipa, Moragas-Tellis, Carla Junqueira, Costa, Rui Miguel Gil da, da Silva, Marcos Antonio Custódio Neto, Almeida-Souza, Fernando, Silva Calabrese, Kátia da, Azevedo-Santos, Ana Paula Silva, Nascimento, Maria do Desterro Soares Brandão
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 29.10.2020; MDPI AG
• Subjects: Animals; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antineoplastic Agents - toxicity; antitumor; Carcinoma, Ehrlich Tumor - drug therapy; Carcinoma, Ehrlich Tumor - immunology; Carcinoma, Ehrlich Tumor - metabolism; Carcinoma, Ehrlich Tumor - pathology; Cytokines - metabolism; Ehlich’s tumor; Female; inflammation; Inflammation Mediators - metabolism; meroterpenoids; Mice; Molecular Structure; P. purpurogenum; Talaromyces - metabolism; Tumor Burden - drug effects; Water Microbiology; Ehlich’s tumor; antitumor; inflammation; meroterpenoids; P. purpurogenum
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md18110541

This study addresses the antitumoral properties of isolated from a polluted lagoon in Northeastern Brazil. Ethyl Acetate Extracellular Extract (EAE) was used. The metabolites were studied using direct infusion mass spectrometry. The solid Ehrlich tumor model was used for antitumor activity. Female Swiss mice were divided into groups ( = 10/group) as follows: The negative control (CTL-), treated with a phosphate buffered solution; the positive control (CTL+), treated with cyclophosphamide (25 mg/kg); extract treatments at doses of 4, 20, and 100 mg/kg; animals without tumors or treatments (Sham); and animals without tumors treated with an intermediate dose (EAE20). All treatments were performed intraperitoneally, daily, for 15 days. Subsequently, the animals were euthanized, and the tumor, lymphoid organs, and serum were used for immunological, histological, and biochemical parameter evaluations. The extract was rich in meroterpenoids. All doses significantly reduced tumor size, and the 20 and 100 mg/kg doses reduced tumor-associated inflammation and tumor necrosis. The extract also reduced the cellular infiltration of lymphoid organs and circulating TNF-α levels. The extract did not induce weight loss or renal and hepatic toxic changes. These results indicate that exhibits immunomodulatory and antitumor properties in vivo. Thus, fungal fermentation is a valid biotechnological approach to the production of antitumor agents.