Campylobacter Phage Isolation and Characterization: What We Have Learned So Far

Lytic phages, which can be used to combat this pathogen in animals and on food products, have been studied for more than 30 years. Though, due to some peculiarities of the phages, which hampered their isolation and particularly their molecular analysis for a long time, progress in this research fiel...

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Published inMethods and protocols Vol. 2; no. 1; p. 18
Main Authors Jäckel, Claudia, Hammerl, Jens Andre, Hertwig, Stefan
Format Journal Article
LanguageEnglish
Published Switzerland MDPI 15.02.2019
MDPI AG
Subjects
bacteriophage (phage)
Campylobacter
genome
host range
isolation
propagation
Review
sequencing
genome
propagation
bacteriophage (phage)
isolation
host range
Campylobacter
sequencing
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Summary:Lytic phages, which can be used to combat this pathogen in animals and on food products, have been studied for more than 30 years. Though, due to some peculiarities of the phages, which hampered their isolation and particularly their molecular analysis for a long time, progress in this research field was rather slow. Meanwhile, the situation has changed and much more is known about the biology and genetics of those phages. In this article, we address specific issues that should be considered when phages are studied, starting with the isolation and propagation of the phages and ending with a thorough characterization including whole-genome sequencing. The basis for advice and recommendations given here is a careful review of the scientific literature and experiences that we have had ourselves with phages.
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Both authors contributed equally to this work.
ISSN:2409-9279
2409-9279
DOI:10.3390/mps2010018