Study on optimization of extraction process and resistance to oxidation of Polypeptide from sea cucumber waste liquid

The protease hydrolysis technology is used to extract the polypeptides from the sea cucumber waste liquid. The enzymatic hydrolysis ability of protein in sea cucumber waste liquid by seven proteases shows that the best protease is compound protease by taking the hydrolysis degree as the index. Furth...

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Published inIOP conference series. Earth and environmental science Vol. 559; no. 1; pp. 12025 - 12032
Main Authors Wang, Shou-Quan, Liu, Fa-Xin, Wu, Jing, Yin, Feng-Jiao, Shi, Yong-Chun
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.08.2020
Subjects
Antioxidants
Dosage
Enzymes
Hydrolysis
Optimization
Oxidation
Oxidation resistance
pH effects
Polypeptides
Protease
Proteinase
Proteins
Response surface methodology
Scavenging
Surface analysis (chemical)
Vegetables
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Summary:The protease hydrolysis technology is used to extract the polypeptides from the sea cucumber waste liquid. The enzymatic hydrolysis ability of protein in sea cucumber waste liquid by seven proteases shows that the best protease is compound protease by taking the hydrolysis degree as the index. Furthermore, the effects of enzyme dosage, pH, temperature and time of enzymatic hydrolysis on the hydrolysis degree are studied, and Box-Benhnken central composite test and response surface analysis method are used to optimize the enzymatic hydrolysis conditions.. The results show that the optimal enzymatic hydrolysis conditions are: enzyme dosage: 647 U/g; pH: 6.48; temperature: 52.5°C; enzymatic hydrolysis time: 3.44h; protein hydrolysis degree under the best condition: 25.9%. The polypeptides extracted from the sea cucumber waste liquid have high antioxidant activity, and the DPPH· scavenging activity increases with the polypeptide concentration.
ISSN:1755-1307
1755-1315
DOI:10.1088/1755-1315/559/1/012025