Infrared videomicroscopy: a new look at neuronal structure and function
Brain slices were introduced as a standard preparation for neurophysiological experiments some 20 years ago. A drawback of this preparation compared with cell culture has been the difficulty to visualize individual neurones in standard thick slices. This problem has been overcome by the use of infra...
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Published in | Trends in neurosciences (Regular ed.) Vol. 17; no. 11; pp. 453 - 458 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
01.11.1994
Elsevier Science Elsevier Sequoia S.A |
Subjects | |
Online Access | Get full text |
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Summary: | Brain slices were introduced as a standard preparation for neurophysiological experiments some 20 years ago. A drawback of this preparation compared with cell culture has been the difficulty to visualize individual neurones in standard thick slices. This problem has been overcome by the use of infrared videomicroscopy. Neurones in slices can now be visualized in great detail, and neuronal processes can be patch-clamped under direct visual control. Infrared videomicroscopy has also been applied successfully to other fields of neuroscience such as neuronal development and neurotoxicity. A further development of infrared videomicroscopy enables one to visualize the spread of excitation in slices making the technique a tool for the direct investigation of neuronal function. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 ObjectType-Review-3 content type line 23 |
ISSN: | 0166-2236 1878-108X |
DOI: | 10.1016/0166-2236(94)90130-9 |