The hexahistidine containing organophosphorus hydrolase enzyme and bacterial cellulose based functional materials

Bacterial cellulose (BC) is a unique biopolymer for creation on its basis functional materials used as drugs, matrices for regenerative therapy, dressings, etc. In this study, the possibility of combining of BC with hexahistidine containing organophosphorus hydrolase (His6-OPH), exhibiting lactonase...

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Bibliographic Details
Published inIOP conference series. Materials Science and Engineering Vol. 525; no. 1; pp. 12005 - 12010
Main Authors Aslanli, A G, Stepanov, N A, Senko, O V, Maslova, O V, Lyagin, I V, Efremenko, E N
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.01.2019
Subjects
Antiinfectives and antibacterials
Antimicrobial agents
Bacteria
Biopolymers
Cellulose
Composite materials
Composition
Enzymes
Functional materials
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Summary:Bacterial cellulose (BC) is a unique biopolymer for creation on its basis functional materials used as drugs, matrices for regenerative therapy, dressings, etc. In this study, the possibility of combining of BC with hexahistidine containing organophosphorus hydrolase (His6-OPH), exhibiting lactonase activity against gram-negative pathogenic bacteria is considered to obtain composite materials with special properties. The dry and wet samples of fibrous BC, impregnated with solutions of the His6-OPH enzyme and various antimicrobial agents were obtained; their catalytic characteristics were investigated. The preservation of the enzymatic activity of the His6-OPH enzyme in the composition of the obtained samples with BC, including after the lyophilization process, has been established. The stabilization of the enzymatic activity of His6-OPH in the composition of fibrous materials in the presence of poly(aspartic) acid and antimicrobial agents is shown.
ISSN:1757-8981
1757-899X
1757-899X
DOI:10.1088/1757-899X/525/1/012005