Heterotrophic nitrification and aerobic denitrification by a novel Acinetobacter sp. TAC-1 at low temperature and high ammonia nitrogen

• Published in: Bioresource technology Vol. 339; p. 125620
• Main Authors: Zhao, Tiantao, Chen, Peipei, Zhang, Lijie, Zhang, Lei, Gao, Yanhui, Ai, Shuo, Liu, Hao, Liu, Xiangyang
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.11.2021
• Subjects: Acinetobacter; Aerobic denitrification; ammonium nitrogen; Cold-responsive gene; denitrification; genes; Heterotrophic nitrification; Low temperature; nitrate nitrogen; nitrate reductase; nitrification; nitrite nitrogen; nitrite reductase; nitrogen; Nitrogen removal pathway; phenotype; phylogeny; technology; temperature; transcriptome; transport proteins; Heterotrophic nitrification; Nitrogen removal pathway; Cold-responsive gene; Aerobic denitrification; Low temperature
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2021.125620

[Display omitted] •A novel HN-AD bacteria were isolated from swine wastewater.•It had excellent HN-AD function at 5 °C.•It can tolerate high concentrations of ammonium, nitrate and nitrite nitrogen.•The nitrogen metabolism pathway of TAC-1 strain was revealed.•Its cold tolerance is closely related to membrane transport and membrane stability. A novel strain was isolated from swinewastewater and identified as Acinetobacter sp. TAC-1 based on its phylogenetic and phenotypic characteristics. The strain TAC-1 was found to have a high ability to metabolize ammonium-N under low temperature condition. The strain TAC-1 could remove approximately 94.6% of ammonium-N (400 mg/L), 93.3% of nitrate-N (400 mg/L) and 42.4% of nitrite-N (400 mg/L) at 5 °C. The functional genes nitrate reductase gene (narG) and nitrite reductase gene (nirK, nirS) were successfully amplified by qPCR, further evidencing the heterotrophic nitrification and aerobic denitrification capability of Acinetobacter sp. TAC-1. The transcriptome data confirmed that the membrane transport protein and unsaturated fatty acid dehydrogenase-related genes of the strain TAC-1 were significantly up-regulated at 5 °C, enabling it to survive low temperatures. The high nitrogen removal ability at 5 °C makes this strain have a good application prospect.