Restriction fragment length polymorphism analysis of major histocompatibility complex genes in the non-obese diabetic mouse strain and its non-diabetic sister strains

• Published in: Diabetologia Vol. 32; no. 2; p. 118
• Main Authors: Fujishima, Y, Koide, Y, Kaidoh, T, Nishimura, M, Yoshida, T O
• Format: Journal Article
• Language: English
• Published: Germany 01.02.1989
• Subjects: Animals; Blotting, Northern; Blotting, Southern; Chromosome Mapping; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Experimental - immunology; Genes, MHC Class I; Genes, MHC Class II; Major Histocompatibility Complex; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred ICR; Mice, Inbred Strains - immunology; Mice, Mutant Strains; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Species Specificity
• ISSN: 0012-186X
• DOI: 10.1007/bf00505184

It has been suggested that one of the recessive genes controlling diabetes in non-obese diabetic mice is linked to the major histocompatibility complex. We, therefore, performed restriction fragment length polymorphism studies of major histocompatibility complex genes (class I, II, and III) in non-obese diabetic mice in comparison with those of their non-diabetic sister strains, non-obese non-diabetic, cataract, and ILI mice which were derived from the same Jcl-ICR mice as the non-obese diabetic mouse was. When class II and III probes and a minimum of four restriction enzymes were used, class II and III genes of non-obese diabetic mice were indistinguishable from those of cataract and ILI mice but totally different from those of non-obese non-diabetic mice. The studies also indicated that A beta, E beta, and C4-Slp genes of non-obese diabetic, cataract, and ILI mice, and A alpha, A beta, E beta and C4-Slp genes of non-obese non-diabetic mice are different from those of BALB/c and C57BL/6 mice, respectively. While non-obese non-diabetic mice expressed the E alpha gene, non-obese diabetic, cataract, and ILI mice appeared to carry a deletion in the 5' end of the E alpha gene resulting in failure to transcribe the E alpha gene. When class I probe was used, cataract mice showed very different band patterns from those of the other ICR-derived mice. It is suggested that non-obese diabetic, non-obese non-diabetic, and ILI mice contain only a single class I D region gene.