Further characterization of monoclonal antibodies to Echinococcus granulosus Antigen 5 and Antigen B

• Published in: International journal for parasitology Vol. 22; no. 3; pp. 391 - 394
• Main Authors: Liu, D., Rickard, M.D., Lightowlers, M.W.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.05.1992; Elsevier Science
• Subjects: Animals; Antibodies, Helminth - immunology; Antibodies, Monoclonal - immunology; Antigen 5; Antigen B; antigenic characteristics; antigenic determinants; antigens; Antigens, Helminth - immunology; Biological and medical sciences; cross reaction; Echinococcus - immunology; Echinococcus granulosus; Enzyme-Linked Immunosorbent Assay; epitopes; Epitopes - immunology; Helminthic diseases; hydatid cyst fluid; hydatids; Infectious diseases; Medical sciences; Miscellaneous; monoclonal antibodies; monoclonal antibody; Parasitic diseases; Sheep; sheep hydatid cyst fluid antigens; hydatid cyst fluid; Antigen B; monoclonal antibody; Echinococcus granulosus; Antigen 5; Antigen; Antigenic determinant; Helmintha; Plathelmintha; Parasite; Antigen antibody reaction; Monoclonal antibody; Cestoda; Invertebrata; ELISA assay; Recognition
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/S0020-7519(05)80019-0

Two monoclonal antibodies (24.14, 61A12) to Echinococcus granulosus Antigen 5 and two (31.15 and 39B3) to Antigen B were further characterized using modified sheep hydatid cyst fluid antigens (SHCF) in ELISA. None of these four monoclonals were directed against carbohydrate or lipid epitopes of SHCF antigens since they all reacted strongly with periodate or lipase-treated SHCF. On the other hand, they appeared to recognize SHCF determinants of protein nature as protease treatment of SHCF destroyed binding with the monoclonals. Anti-Antigen B monoclonals 31.15 and 39B3 showed strong reaction with boiled SHCF and anti-Antigen 5 monoclonal 24.14 did not. However, the second anti-Antigen 5 monoclonal 61A12 also reacted with boiled SHCF suggesting that some epitopes of Antigen 5 are heat stable. 24.14 and 61A12 may recognize a similar epitope of Antigen 5 whereas 39B3 may be against an epitope of Antigen B different from that recognized by 31.15.