Leaf proteome responses of Arabidopsis thaliana exposed to mild cadmium stress

• Published in: Journal of plant physiology Vol. 167; no. 4; pp. 247 - 254
• Main Authors: Semane, Brahim, Dupae, Joke, Cuypers, Ann, Noben, Jean-Paul, Tuomainen, Marjo, Tervahauta, Arja, Kärenlampi, Sirpa, Van Belleghem, Frank, Smeets, Karen, Vangronsveld, Jaco
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 01.03.2010; Elsevier
• Subjects: 2-DE; Analysis of Variance; Arabidopsis - drug effects; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biological and medical sciences; Cadmium; Cadmium - toxicity; chlorosis; Electrophoresis, Gel, Two-Dimensional; enzyme activity; Exposure; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant - drug effects; heavy metals; Leaf proteome; lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Metabolism; Models, Biological; oxidative stress; Oxidative Stress - drug effects; peroxidase; Photosynthesis; physiological response; phytoremediation; plant growth; Plant Leaves - drug effects; Plant Leaves - enzymology; Plant Leaves - metabolism; Plant physiology and development; plant proteins; plant response; plant stress; Proteins; proteome; Proteome - metabolism; seedlings; Stress, Physiological - drug effects; Stresses; two-dimensional gel electrophoresis; Cadmium; IPG; SOD; 2-DE; IEF; MDA; SDS–PAGE; CHAPS; Arabidopsis thaliana; Leaf proteome; PC; TCA; SPOD; DDT; ROS; TBA-rc; GSH; GPOD; Proteome; Plant leaf; Stress; Response; Plant physiology; Cruciferae; Dicotyledones; Angiospermae; Spermatophyta; Experimental plant
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2009.09.015

The leaf proteome of 3-week-old Arabidopsis thaliana seedlings exposed for 1 week to low, environmentally realistic Cd concentrations was investigated. The data indicated that at 1 μM Cd, A. thaliana plants adapted their metabolism to cope with the Cd exposure. As a result, only moderate protein changes were observed. However, at 10 μM Cd, severe stress was indicated by growth reduction and chlorosis of rosette leaves at the macroscopic level and by lipid peroxidation and enhanced peroxidase activity at the cellular level. Of the 730 reproducible proteins among all gels, 21 were statistically upregulated in response to Cd. These proteins can be functionally grouped into 5 classes: proteins involved in (1) oxidative stress response, (2) photosynthesis and energy production, (3) protein metabolism, (4) gene expression and finally, (5) proteins with various or unknown function. In order to provide greater insight into the mechanisms induced on Cd exposure, a working model is proposed.