Oocydin A, a chlorinated macrocyclic lactone with potent anti- oomycete activity from Serratia marcescens

• Published in: Microbiology (Society for General Microbiology) Vol. 145; no. 12; pp. 3557 - 3564
• Main Authors: Strobel, Gary, Li, Jia-Yao, Sugawara, Fumio, Koshino, Hiroyuki, Harper, James, Hess, W. M
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.12.1999; Society for General Microbiology
• Subjects: Animal, plant and microbial ecology; Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Bacteriology; Biological and medical sciences; Chromatography, High Pressure Liquid; Culture Media; Ecosystem; Fresh Water; Fundamental and applied biological sciences. Psychology; Lactones - chemistry; Lactones - isolation & purification; Lactones - metabolism; Lactones - pharmacology; macrocyclic lactone; Mass Spectrometry - methods; Microbial ecology; Microbial Sensitivity Tests; Microbiology; Microflora of plants; Microscopy, Electron, Scanning; oocydin A; Oomycetes - drug effects; Oomycetes - growth & development; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phytophthora - drug effects; Phytophthora - growth & development; Plants - microbiology; Rhyncholacis pedicillata; Serratia marcescens; Serratia marcescens - growth & development; Serratia marcescens - metabolism; South America; Oocydin A; Purification; Plant pathogen; Molecular structure; Biological control; Epiphyte; Phycomycetes; Vegetal microorganism relation; Lactone; Biological activity; Fungi; Freshwater environment; Antifungal agent; Pythium ultimum; Minimum inhibitory concentration; Serratia marcescens; Bacteria; Phytophthora; Enterobacteriaceae; Thallophyta
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-145-12-3557

Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA 1 Department of Applied Biological Science, Science University of Tokyo, Noda, Chiba 278-8510, Japan 2 The Institute of Physical and Chemical Research, Wako, Saitama 351- 0198, Tokyo, Japan 3 Department of Chemistry, University of Utah, Salt Lake City, UT 84112, USA 4 Department of Botany and Range Science, Brigham Young University, Provo, UT 84602, USA 5 Author for correspondence: Gary Strobel. Tel: +1 406 994 5148. Fax: +1 406 994 7600. e-mail: uplgs{at}montana.edu A unique chlorinated macrocyclic lactone, termed oocydin A, was isolated from a strain of Serratia marcescens growing as an epiphyte on Rhyncholacis pedicillata , an aquatic plant native to the Carrao river of the Venezuelan-Guyanan region of South America. The lactone has a molecular mass of 470 Da, and contains one atom of chlorine, a carboxyl group and a tetrahydrofuran ring internal to a larger macrocyclic ring. MICs of approximately 0·03 µg ml -1 were noted for oocydin A against such phytopathogenic oomycetes as Pythium ultimum, Phytophthora parasitica , Phytophthora cinnamomi and Phytophthora citrophora . With regard to the true fungi, oocydin A had either minimal or no effect against certain Fungi Imperfecti (including several pathogens of humans), two ascomycetes and a basidiomycete. Oocydin A may have potential as an antimycotic in agricultural applications and especially for crop protection. Keywords: antimycotic, phycomycetes, aquatic ecology , epiphyte