Identification of Mycoplasmas Using a Fluorophore-Free Microarray and Infrared Chemical Imaging (IRCI)

A novel application of mid-infrared chemical imaging (IRCI) for the fluorophore-free detection and identification of mycoplasma species is reported for the first time. The PCR-amplified biotinylated targets hybridized to microarray probes were treated with streptavidin-gold nanoparticles followed by...

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Published inJournal of microbiological methods Vol. 86; no. 3; pp. 383 - 386
Main Authors Mossoba, M.M., Chizhikov, V., Volokhov, D.V., Martinez-Diaz Milians, K., Schoen, B., Al-Khaldi, S.F.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.09.2011
Elsevier
Subjects
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
DNA microarrays
DNA, Bacterial - analysis
DNA, Bacterial - chemistry
Fundamental and applied biological sciences. Psychology
Gold - chemistry
image analysis
Infrared Imaging
Infrared Rays
Metal Nanoparticles - chemistry
microarray technology
Microbiology
Mycoplasma
Mycoplasma - genetics
Mycoplasma - isolation & purification
Mycoplasma detection
nanoparticles
Oligonucleotide Array Sequence Analysis - methods
Polymerase Chain Reaction - methods
silver
Silver - chemistry
Spectrophotometry, Infrared
Streptavidin - chemistry
Mycoplasma detection
Infrared Imaging
DNA microarrays
Mollicutes
Mycoplasmatales
DNA chip
Bacteria
Identification
Detection
Mycoplasma
Mycoplasmataceae
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Abstract A novel application of mid-infrared chemical imaging (IRCI) for the fluorophore-free detection and identification of mycoplasma species is reported for the first time. The PCR-amplified biotinylated targets hybridized to microarray probes were treated with streptavidin-gold nanoparticles followed by silver enhancement. This modification has the potential to expand the implementation of DNA microarray techniques in laboratories involved in the detection of cell substrates, other biological products, and clinical materials for the presence of mycoplasmas.
AbstractList A novel application of mid-infrared chemical imaging (IRCI) for the fluorophore-free detection and identification of mycoplasma species is reported for the first time. The PCR-amplified biotinylated targets hybridized to microarray probes were treated with streptavidin-gold nanoparticles followed by silver enhancement. This modification has the potential to expand the implementation of DNA microarray techniques in laboratories involved in the detection of cell substrates, other biological products, and clinical materials for the presence of mycoplasmas.
Author Schoen, B.
Mossoba, M.M.
Volokhov, D.V.
Chizhikov, V.
Al-Khaldi, S.F.
Martinez-Diaz Milians, K.
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Issue 3
Keywords Mycoplasma detection
Infrared Imaging
DNA microarrays
Mollicutes
Mycoplasmatales
DNA chip
Bacteria
Identification
Detection
Mycoplasma
Mycoplasmataceae
Language English
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Snippet A novel application of mid-infrared chemical imaging (IRCI) for the fluorophore-free detection and identification of mycoplasma species is reported for the...
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StartPage 383
SubjectTerms Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
DNA microarrays
DNA, Bacterial - analysis
DNA, Bacterial - chemistry
Fundamental and applied biological sciences. Psychology
Gold - chemistry
image analysis
Infrared Imaging
Infrared Rays
Metal Nanoparticles - chemistry
microarray technology
Microbiology
Mycoplasma
Mycoplasma - genetics
Mycoplasma - isolation & purification
Mycoplasma detection
nanoparticles
Oligonucleotide Array Sequence Analysis - methods
Polymerase Chain Reaction - methods
silver
Silver - chemistry
Spectrophotometry, Infrared
Streptavidin - chemistry
Title Identification of Mycoplasmas Using a Fluorophore-Free Microarray and Infrared Chemical Imaging (IRCI)
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