Identification of Mycoplasmas Using a Fluorophore-Free Microarray and Infrared Chemical Imaging (IRCI)

• Published in: Journal of microbiological methods Vol. 86; no. 3; pp. 383 - 386
• Main Authors: Mossoba, M.M., Chizhikov, V., Volokhov, D.V., Martinez-Diaz Milians, K., Schoen, B., Al-Khaldi, S.F.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.2011; Elsevier
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; DNA microarrays; DNA, Bacterial - analysis; DNA, Bacterial - chemistry; Fundamental and applied biological sciences. Psychology; Gold - chemistry; image analysis; Infrared Imaging; Infrared Rays; Metal Nanoparticles - chemistry; microarray technology; Microbiology; Mycoplasma; Mycoplasma - genetics; Mycoplasma - isolation & purification; Mycoplasma detection; nanoparticles; Oligonucleotide Array Sequence Analysis - methods; Polymerase Chain Reaction - methods; silver; Silver - chemistry; Spectrophotometry, Infrared; Streptavidin - chemistry; Mycoplasma detection; Infrared Imaging; DNA microarrays; Mollicutes; Mycoplasmatales; DNA chip; Bacteria; Identification; Detection; Mycoplasma; Mycoplasmataceae
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2011.06.003

A novel application of mid-infrared chemical imaging (IRCI) for the fluorophore-free detection and identification of mycoplasma species is reported for the first time. The PCR-amplified biotinylated targets hybridized to microarray probes were treated with streptavidin-gold nanoparticles followed by silver enhancement. This modification has the potential to expand the implementation of DNA microarray techniques in laboratories involved in the detection of cell substrates, other biological products, and clinical materials for the presence of mycoplasmas.