Interaction of phase singularities on the spiral wave tail: reconsideration of capturing the excitable gap

• Published in: American journal of physiology. Heart and circulatory physiology Vol. 315; no. 2; pp. H318 - H326
• Main Authors: Tomii, Naoki, Yamazaki, Masatoshi, Arafune, Tatsuhiko, Kamiya, Kaichiro, Nakazawa, Kazuo, Honjo, Haruo, Shibata, Nitaro, Sakuma, Ichiro
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.08.2018
• Subjects: Anatomy & physiology; Collision dynamics; Excitation; Heart attacks; Laboratory animals; Rabbits; Refractory period; Shock waves; Singularities; Stimulation; Variance analysis; tachyarrhythmia; phase singularity; optical mapping; defibrillation; spiral wave
• ISSN: 0363-6135; 1522-1539
• DOI: 10.1152/ajpheart.00558.2017

The action mechanism of stimulation toward spiral waves (SWs) owing to the complex excitation patterns that occur just after point stimulation has not yet been experimentally clarified. This study sought to test our hypothesis that the effect of capturing excitable gap of SWs by stimulation can also be explained as the interaction of original phase singularity (PS) and PSs induced by the stimulation on the wave tail (WT) of the original SW. Phase variance analysis was used to quantitatively analyze the postshock PS trajectories. In a two-dimensional subepicardial layer of Langendorff-perfused rabbit hearts, optical mapping was used to record the excitation pattern during stimulation. After a SW was induced by S1-S2 shock, single biphasic point stimulation S3 was applied. In 70 of the S1-S2-S3 stimulation episodes applied on 6 hearts, the original PS was clearly observed just before the S3 point stimulation in 37 episodes. Pairwise PSs were newly induced by the S3 in 20 episodes. The original PS collided with the newly induced PSs in 16 episodes; otherwise, they did not interact with the original PS. SW shift occurred most efficiently when the S3 shock was applied at the relative refractory period, and PS shifted in the direction of the WT. In conclusion, quantitative tracking of PS clarified that stimulation in desirable conditions induces pairwise PSs on WT and that the collision of PSs causes SW shift along the WT. The results of this study indicate the importance of the interaction of shock-induced excitation with the WT for effective stimulation. NEW & NOTEWORTHY The quantitative analysis of spiral wave dynamics during stimulation clarified the action mechanism of capturing the excitable gap, i.e., the induction of pairwise phase singularities on the wave tail and spiral wave shift along the wave tail as a result of these interactions. The importance of the wave tail for effective stimulation was revealed.