Spectral focusing in picosecond pulsed stimulated Raman scattering microscopy

We introduce spectral focusing of picosecond laser pulses in stimulated Raman scattering (SRS) microscopy to improve spectral resolution, reduce nonlinear background signals, and decrease nonlinear photodamage. We produce a pair of 14 ps pump and Stokes laser pulses by spectral focusing of a 2 ps la...

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Bibliographic Details
Published inBiomedical optics express Vol. 13; no. 2; pp. 995 - 1004
Main Authors Koike, Kota, Smith, Nicholas I, Fujita, Katsumasa
Format Journal Article
LanguageEnglish
Published United States Optica Publishing Group 01.02.2022
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Summary:We introduce spectral focusing of picosecond laser pulses in stimulated Raman scattering (SRS) microscopy to improve spectral resolution, reduce nonlinear background signals, and decrease nonlinear photodamage. We produce a pair of 14 ps pump and Stokes laser pulses by spectral focusing of a 2 ps laser and achieve a spectral resolution of 2 cm . Due to instantaneous narrow-band excitation, we find that the chirped 14 ps laser pulses can be used to improve the signal-to-background ratio in SRS microscopy of various samples such as polymer particles and small molecules in HeLa cells. The lower peak powers produced by chirped picosecond laser pulses also reduce nonlinear photodamage, allowing long-term SRS imaging of living cells with higher SNR.
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ISSN:2156-7085
2156-7085
DOI:10.1364/boe.445640