Structural insights into the EthR-DNA interaction using native mass spectrometry

EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR-DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to...

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Published inChemical communications (Cambridge, England) Vol. 53; no. 25; pp. 3527 - 3530
Main Authors Shiu-Hin Chan, Daniel, Seetoh, Wei-Guang, McConnell, Brendan N, Matak-Vinković, Dijana, Thomas, Sherine E, Mendes, Vitor, Blaszczyk, Michal, Coyne, Anthony G, Blundell, Tom L, Abell, Chris
Format Journal Article
LanguageEnglish
Published England 23.03.2017
Subjects
Calorimetry
DNA, Bacterial - chemistry
DNA, Bacterial - metabolism
Drug Resistance, Bacterial
Ethionamide - chemistry
Ethionamide - metabolism
Mass spectrometry
Mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Operators
Spectrometry, Mass, Electrospray Ionization
Thermodynamics
Tuberculosis
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Summary:EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR-DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to bind to its operator.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc00804j