Evaluation of arabica coffee propagation using cell suspension culture

• Published in: IOP conference series. Earth and environmental science Vol. 418; no. 1; pp. 12013 - 12019
• Main Authors: Ibrahim, M S D, Tresniawati, C
• Format: Journal Article
• Language: English
• Published: Bristol IOP Publishing 01.01.2020
• Subjects: Casein; Cell culture; Coffee; Embryogenesis somatic; Embryonic growth stage; Embryos; Genotypes; Germination; hyperhydricity; liqluid culture; Media; plant genotype; Propagation; Regeneration; Seedlings; solid culture; Somatic embryogenesis; Sucrose; Suspension culture
• ISSN: 1755-1307; 1755-1315
• DOI: 10.1088/1755-1315/418/1/012013

Arabica coffee is highly produced in Indonesia, thus need to be propagated using a powerful approach like cell culture in somatic embryogeneis for providing seedlings. The study aimed to evaluate the use of cell cultures in somatic embryogenesis on several Arabica coffee genotypes. The embryogenic calli (200 mg) were subcultured into solid and liquid regeneration media (half concentration of Murashige and Skoog [MS] added with 2 mg/L of kinetine, 35 g/L of sucrose, 400 mg/L of casein hydrolisate, and mall extract). For the somatic embryos germination, MS media was used by adding it with 0.3 mg/L of BAP and 40 g/L of sucrose. The solid media was added with 2.5 g/L of phytagel. This study was used Factorial Completely Randomized Design (CRD) (genotype and media solidity) with 10 replications.. The results showed that production of the number of somatic embryos is highly depended on the genotypes. The size of somatic embryos, fresh weight, and root length on the torpedo and germination phases in liquid media was higher than solid media. Using liquid media at germination phase isn't recommended, because it can resulted hyperhydricity.