Nonrandom 4p13 rearrangements of the RhoH/TTF gene, encoding a GTP-binding protein, in non-Hodgkin's lymphoma and multiple myeloma

• Published in: Oncogene Vol. 19; no. 16; pp. 2023 - 2032
• Main Authors: PREUDHOMME, C, ROUMIER, C, KERCKAERT, J. P, GALIEGUE-ZOUITINA, S, HILDEBRAND, M. P, DALLERY-PRUDHOMME, E, LANTOINE, D, LAÏ, J. L, DAUDIGNON, A, ADENIS, C, BAUTERS, F, FENAUX, P
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 13.04.2000; Nature Publishing Group
• Subjects: Base Sequence; Bcl-6 protein; BCL6 gene; Biological and medical sciences; Blotting, Northern; chromosome 14; Chromosome 3; Chromosome 4; Chromosomes, Human, Pair 3; Chromosomes, Human, Pair 4; DNA-Binding Proteins - genetics; Female; Fluorescence in situ hybridization; Gene mapping; Gene Rearrangement; Genes; GTP-binding protein; GTP-Binding Proteins - genetics; Heavy chains; Hematologic and hematopoietic diseases; Humans; Immunoglobulin Heavy Chains - genetics; Immunoglobulins; In Situ Hybridization, Fluorescence; LAZ3 gene; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma; Lymphoma, Non-Hodgkin - genetics; Medical sciences; Middle Aged; Molecular Sequence Data; Multiple myeloma; Multiple Myeloma - genetics; Non-Hodgkin's lymphoma; Patients; Proteins; Proto-Oncogene Proteins c-bcl-6; Reverse Transcriptase Polymerase Chain Reaction; Rho protein; RhoH gene; RhoH/TTF gene; Translocation, Genetic; TTF gene; France; Chromosomal aberration; GTP; Genome organization; Molecular interaction; Chromosome B4; Myeloma; Binding protein; Signal transduction; Immunoglobulinopathy; Gene; Lymphoproliferative syndrome; Fluorescence in situ hybridization; G protein; Genetic mapping; Human; Translocation; Immunopathology; Burkitt lymphoma; Malignant hemopathy; Gene expression; Non Hodgkin lymphoma; Infection; Cell line; Viral disease; Gene rearrangement
• ISSN: 0950-9232; 1476-5594
• DOI: 10.1038/sj.onc.1203521

We recently isolated the RhoH/TTF gene by its fusion to the LAZ3/BCL6 gene, in a non-Hodgkin's lymphoma (NHL) cell line, which bore a t(3;4)(q27;p11-13) translocation. This gene encodes a novel Rho GTP-binding protein and is specifically expressed in hematopoietic tissues. We made its precise mapping at band 4p13, and described its partial genomic structure. Using fluorescence in situ hybridization and molecular analyses, we report here on the rearrangement of the RhoH/TTF gene, at band 4p13, in four cases of NHL with t(3;4)(q27;p13) translocation and its fusion to the LAZ3/BCL6 gene at band 3q27, in three of these cases. RT-PCR analysis of two cases allowed the detection of variable fusion transcripts emerging from the rearranged alleles, and in one case, a deregulated expression of both RhoH/TTF and LAZ3/BCL6 genes, by promoter substitution, was observed. We also show here another rearrangement of the RhoH/TTF gene in a patient with multiple myeloma and t(4;14)(p13;q32) translocation, with breakage within the IGH gene. It is the first report which describes the recurrent chromosomal alteration of a GTP-binding protein encoding gene, in patients with hematopoietic malignancies.