Exploitation of De Novo helper-lipids for effective gene delivery

• Published in: Journal of pharmacy & pharmaceutical sciences Vol. 11; no. 4; p. 56
• Main Authors: Kurosaki, Tomoaki, Kitahara, Takashi, Teshima, Mugen, Nishida, Koyo, Nakamura, Junzo, Nakashima, Mikiro, To, Hideto, Hukuchi, Hiromitsu, Hamamoto, Tomoyuki, Sasaki, Hitoshi
• Format: Journal Article
• Language: English
• Published: Canada Frontiers Media S.A 01.01.2008
• Subjects: Animals; Cell Line; Disease Models, Animal; Erythrocytes - metabolism; Gene Transfer Techniques; Genetic Therapy; Genetic Vectors; Humans; Lipids - chemistry; Liposomes - metabolism; Liposomes - pharmacokinetics; Mice; Phosphatidylethanolamines - chemistry; Quaternary Ammonium Compounds - chemistry; Transfection - methods; Tumor Cells, Cultured
• ISSN: 1482-1826; 1482-1826
• DOI: 10.18433/j31s3b

In gene delivery, a fusogenic lipid such as dioleyl phosphatidylethanolamine (DOPE) which is a component of cationic liposomal vector is important factor for effective transfection efficiency. We investigated the effect of penetration enhancers as alternative helper-lipids to DOPE. Transdermal penetraion enhancers such as N-lauroylsarcosine (LS), (R)-(+)-limonene (LM), vitamin E (VE), and phosphatidyl choline from eggs (EggPC) were used in this experiments as helper-lipids with N-[1-(2, 3-dioleyloxy) propyl]-N, N, N-trimethlylammonium chloride (DOTMA) and cholesterol (CHOL). We examined in vitro transfection efficiency, cytotoxicity, hematotoxicity, and in vivo transfection efficiency of plasmid DNA/cationic liposomes complexes. In transfection experiments in vitro, the cationic lipoplexes containing LS had highest transfection efficiency among the other lipoplexes independently of FBS. Furthermore, the lipoplexes containing LS had lowest cell toxicity among the other lipoplexes in the presence of FBS. As the results of erythrocytes interaction experiment, DOTMA/LS/CHOL, DOTMA/VE/CHOL, and DOTMA/EggPC/CHOL lipoplexes showed extremely lower hematotoxicity. On the basis of these results, the in vivo transfection efficiencies of the lipoplexes were examined. The lipoplexes containing LS had the highest transfection activity among the other lipoplexes. In conclusion, several transdermal penetration enhancers are available for alternative helper-lipids to DOPE in cationic liposomal vectors. Among them, DOTMA/LS/CHOL lipoplexes showed superior characteristics in in vitro transfection efficiency, cell toxicity, hematotoxicity, and in vivo transfection efficiency.