cGMP is required for seed germination in Arabidopsis thaliana

• Published in: Journal of plant physiology Vol. 167; no. 11; pp. 885 - 889
• Main Authors: Teng, Yao, Xu, Wenzhong, Ma, Mi
• Format: Journal Article
• Language: English
• Published: Munich Elsevier GmbH 15.07.2010; Elsevier
• Subjects: 2',3'-cyclic-nucleotide phosphodiesterase; abscisic acid; Abscisic acid (ABA); Abscisic Acid - pharmacology; Aminoquinolines - pharmacology; Arabidopsis - drug effects; Arabidopsis - physiology; Arabidopsis thaliana; biochemical pathways; Biological and medical sciences; Blocking; Carbolines - pharmacology; cGMP; cyclic GMP; Cyclic GMP - pharmacology; enzyme inhibition; Enzyme Inhibitors - pharmacology; Fundamental and applied biological sciences. Psychology; Genetic algorithms; Germination; Germination - drug effects; Gibberellic acid; Gibberellic acid (GA); gibberellins; Gibberellins - pharmacology; guanylate cyclase; Guanylate Cyclase - antagonists & inhibitors; Inhibitors; Phosphodiesterase Inhibitors - pharmacology; phosphoric diester hydrolases; Physiology; Plant physiology and development; Plants (organisms); seed germination; Seeds; Seeds - drug effects; Seeds - physiology; Tadalafil; Triazoles - pharmacology; ABA; NO; BRs; GTP; NP; db-cGMP; Abscisic acid (ABA); Germination; PDEs; cGMP; pGC; PKG; LY 83583; sGC; Arabidopsis thaliana; Gibberellic acid (GA); cNMPs; ICL; GCs; GA; Seeds; Gibberellin; Plant physiology; Cruciferae; Dicotyledones; Sesquiterpenes; Angiospermae; Abscisic acid; Plant growth substance; Spermatophyta; Gibberellic acid; Experimental plant
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2010.01.015

Cyclic guanosine 3′,5′-monophosphate (cGMP) is an important second messenger in animals, and is emerging as a player in regulatory functions in plants. In this study, we investigated the role of cGMP in seed germination in Arabidopsis thaliana (Col-0). We demonstrated that both, a membrane-permeant analogue of cGMP (8-Br-cGMP) and the cyclic nucleotide phosphodiesterase (PDE) inhibitor Tadalafil promoted A. thaliana seed germination, whereas the guanylate cyclase inhibitor LY 83583 (6-anilino-5,8-quinolinedione; LY) inhibited it. LY blocked gibberellic acid (GA)-induced seed germination, whereas GA and 8-Br-cGMP co-treatment increased the germination rate and more effectively overcame LY-inhibition than 8-Br-cGMP alone. The gibberellin biosynthesis inhibitor paclobutrazol (PAC) also blocked 8-Br-cGMP and Tadalafil promotion of seed germination. Furthermore, 8-Br-cGMP and Tadalafil decreased abscisic acid (ABA) sensitivity during seed germination. These findings highlight that cGMP is a positive regulator and plays a crucial role in Arabidopsis seed germination. Furthermore, both GA and cGMP are required for seed germination.