Recombinant human betacellulin promotes the neogenesis of beta-cells and ameliorates glucose intolerance in mice with diabetes induced by selective alloxan perfusion
Recombinant human betacellulin promotes the neogenesis of beta-cells and ameliorates glucose intolerance in mice with diabetes induced by selective alloxan perfusion. K Yamamoto , J Miyagawa , M Waguri , R Sasada , K Igarashi , M Li , T Nammo , M Moriwaki , A Imagawa , K Yamagata , H Nakajima , M Na...
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Published in | Diabetes (New York, N.Y.) Vol. 49; no. 12; pp. 2021 - 2027 |
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Main Authors | , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Alexandria, VA
American Diabetes Association
01.12.2000
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Subjects | |
Online Access | Get full text |
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Summary: | Recombinant human betacellulin promotes the neogenesis of beta-cells and ameliorates glucose intolerance in mice with diabetes
induced by selective alloxan perfusion.
K Yamamoto ,
J Miyagawa ,
M Waguri ,
R Sasada ,
K Igarashi ,
M Li ,
T Nammo ,
M Moriwaki ,
A Imagawa ,
K Yamagata ,
H Nakajima ,
M Namba ,
Y Tochino ,
T Hanafusa and
Y Matsuzawa
Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, Suita, Japan.
Abstract
Betacellulin (BTC), a member of the epidermal growth factor family, is expressed predominantly in the human pancreas and induces
the differentiation of a pancreatic acinar cell line (AR42J) into insulin-secreting cells, suggesting that BTC has a physiologically
important role in the endocrine pancreas. In this study, we examined the in vivo effect of recombinant human BTC (rhBTC) on
glucose intolerance and pancreatic morphology using a new mouse model with glucose intolerance induced by selective alloxan
perfusion. RhBTC (1 microg/g body wt) or saline was injected subcutaneously every day from the day after alloxan treatment.
The intraperitoneal glucose tolerance test revealed no difference between rhBTC-treated and rhBTC-untreated glucose-intolerant
mice at 2-4 weeks. However, glucose tolerance was significantly improved and body weight was significantly increased in rhBTC-treated
mice compared with untreated mice at 8 weeks. Islet-like cell clusters, consisting mainly of beta-cells, were increased in
the pancreas and were localized in contact with the ductal lining cells and sometimes with acinar cells. In conclusion, administration
of rhBTC improved glucose tolerance in this mouse model by increasing beta-cell volume, primarily through accelerated neogenesis
from ductal lining cells. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0012-1797 1939-327X |
DOI: | 10.2337/diabetes.49.12.2021 |