Regional and temporal expression patterns of interleukin-10, interleukin-10 receptor and adhesion molecules in the rat spinal cord during chronic relapsing EAE

• Published in: Journal of neuroimmunology Vol. 136; no. 1; pp. 94 - 103
• Main Authors: Ledeboer, Annemarie, Wierinckx, Anne, Bol, John G.J.M, Floris, Sarah, Renardel de Lavalette, Chantal, De Vries, Helga E, van den Berg, Timo K, Dijkstra, Christine D, Tilders, Fred J.H, Van Dam, Anne-Marie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2003
• Subjects: Animals; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - immunology; Cell Adhesion Molecules - metabolism; Chemotaxis, Leukocyte - immunology; Chronic Disease; Disease Models, Animal; Encephalomyelitis, Autoimmune, Experimental - immunology; Encephalomyelitis, Autoimmune, Experimental - metabolism; Encephalomyelitis, Autoimmune, Experimental - pathology; Experimental autoimmune encephalomyelitis (EAE); Gene Expression Regulation - genetics; Gene Expression Regulation - immunology; ICAM-1; IL-10; IL-10 receptor; Immunohistochemistry; Inflammation; Intercellular Adhesion Molecule-1 - genetics; Intercellular Adhesion Molecule-1 - immunology; Intercellular Adhesion Molecule-1 - metabolism; Interleukin-10 - genetics; Interleukin-10 - immunology; Interleukin-10 - metabolism; Male; Multiple Sclerosis, Relapsing-Remitting - immunology; Multiple Sclerosis, Relapsing-Remitting - metabolism; Multiple Sclerosis, Relapsing-Remitting - pathology; Rats; Rats, Inbred Strains; Reaction Time - immunology; Receptors, Interleukin - genetics; Receptors, Interleukin - immunology; Receptors, Interleukin - metabolism; Receptors, Interleukin-10; RNA, Messenger - immunology; RNA, Messenger - metabolism; Spinal Cord - immunology; Spinal Cord - metabolism; Spinal Cord - pathology; Time Factors; Vascular Cell Adhesion Molecule-1 - genetics; Vascular Cell Adhesion Molecule-1 - immunology; Vascular Cell Adhesion Molecule-1 - metabolism; VCAM-1; VCAM-1; Experimental autoimmune encephalomyelitis (EAE); Inflammation; ICAM-1; IL-10 receptor; IL-10
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/S0165-5728(03)00031-6

Adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) mediate leukocyte infiltration into the CNS, in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS). Because exogenous interleukin-10 (IL-10) inhibits ICAM-1 and VCAM-1 expression and clinical EAE, we hypothesize that endogenous IL-10 signaling may suppress expression of adhesion molecules. In a rat model of chronic relapsing EAE, expression levels of IL-10 and its receptor (IL-10R1), ICAM-1 and VCAM-1 mRNA in the spinal cord are markedly increased, whereas levels of IL-10 mRNA remain relatively low. The temporal pattern of mRNA and protein expression showed marked differences between spinal cord levels. During relapse, IL-10, IL-10R1, ICAM-1, VCAM-1 mRNA levels and neurological scores show positive correlations. We conclude that endogenous IL-10 is not a crucial factor inhibiting adhesion molecule expression in this model.