Isolation and validation of a candidate Rsv3 gene from a soybean genotype that confers strain-specific resistance to soybean mosaic virus

• Published in: Virology (New York, N.Y.) Vol. 513; pp. 153 - 159
• Main Authors: Tran, Phu-Tri, Widyasari, Kristin, Seo, Jang-Kyun, Kim, Kook-Hyung
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2018
• Subjects: avirulent strains; chromosomes; complementary DNA; crop production; Disease Resistance; Gene Expression; gene overexpression; Gene Silencing; genes; Genes, Plant; Genetic Complementation Test; Genotype; Glycine max; Glycine max (L.) Merr; Glycine max - genetics; Glycine max - immunology; Glycine max - virology; Plant Diseases - immunology; Plant Diseases - virology; Potyvirus - immunology; Resistance gene; Rsv3; Sequence Analysis, DNA; Soybean mosaic virus; soybeans; Rsv3; Soybean mosaic virus; Glycine max (L.) Merr; Resistance gene
• ISSN: 0042-6822; 1096-0341; 1096-0341
• DOI: 10.1016/j.virol.2017.10.014

Soybean mosaic virus (SMV), a member of the genus Potyvirus, significantly reduces soybean production worldwide. Rsv3, which confers strain-specific resistance to SMV, was previously mapped between the markers A519F/R and M3Satt in chromosome 14 of the soybean [Glycine max (L.) Merr.] genotype L29. Analysis of the soybean genome database revealed that five different NBS-LRR sequences exist between the flanking markers. Among these candidate Rsv3 genes, the full-length cDNA of the Glyma.14g204700 was successfully cloned from L29. Over-expression of Glyma.14g204700 in leaves inoculated with SMV inhibited viral infection in a soybean genotype lacking Rsv3. In addition, the transient silencing of the candidate gene caused a high accumulation of an avirulent strain in L29 carrying Rsv3. Our results therefore provide additional line of evidence to support that Glyma.14g204700 is likely Rsv3 gene that confers strain-specific resistance to SMV. •An Rsv3 candidate, Glyma.14g204700, was isolated from soybean genotype L29.•Transient expression of Glyma.14g204700 inhibited SMV-G5H but not SMV-G7H.•Transient silencing of Glyma.14g204700 elevated accumulation of SMV-G5H.