Orostachys japonicus ethyl acetate fraction suppresses MRSA biofilm formation

• Published in: Asian Pacific journal of tropical medicine Vol. 13; no. 1; pp. 38 - 45
• Main Authors: Kim, Jae-Hyeon, Han, Su-Yeon, Kwon, Ji-Hye, Lee, Dong-Seok
• Format: Journal Article
• Language: English
• Published: Wolters Kluwer India Pvt. Ltd 01.01.2020; Department of Smart Foods and Drugs, Graduate School of Inje University, Gimhae 50834, Republic of Korea; Wolters Kluwer Medknow Publications
• Subjects: orostachys japonicus; mrsa; biofilm formation; Biofilm formation; MRSA; Orostachys japonicus
• ISSN: 1995-7645; 2352-4146; 2352-4146
• DOI: 10.4103/1995-7645.273573

Objective: To investigate the effect of Orostachys (O.) japonicus, a perennial herbaceous plant of the Family Crassulaceae, on biofilm formed by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Powdered O. japonicus was extracted by 95% methanol, concentrated, and then, systematically fractionated with n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc), n-butanol, and H2O according to polarity. Among them, the flavonoid-rich EtOAc fraction demonstrated the highest antibacterial activity and was used in this study. Using the biofilm inhibition assay, cell-surface attachment assay, confocal laser scanning microscopy, latex agglutination assay, and real time qRT-PCR, we examined whether the EtOAc fraction inhibited the formation of MRSA biofilm. Results: The EtOAc fraction exhibited distinct activity against biofilm formation and cell-surface attachment of MRSA up to 1 mg/mL through down-regulating the expression of mecA gene and the production and agglutination of penicillin-binding protein 2a as solidly observed in biofilm inhibition assay, cell-suface attachment assay, confocal laser scanning microscopy, latex agglutination assay, and real time qRT-PCR analysis. Conclusions: These results suggest that O. japonicus could be utilized as a potential resource for the development of new anti-biofilm formation of MRSA and antibacterial agents in the future.