miR-34c-5p modulates X-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response

• Published in: The FASEB journal Vol. 33; no. 10; p. 11541
• Main Authors: Bartoszewska, Sylwia, Cabaj, Aleksandra, Dąbrowski, Michał, Collawn, James F, Bartoszewski, Rafal
• Format: Journal Article
• Language: English
• Published: United States 01.10.2019
• Subjects: Apoptosis - genetics; Cell Line, Tumor; Endoplasmic Reticulum - genetics; Endoplasmic Reticulum Stress - genetics; Gene Expression Regulation - genetics; HeLa Cells; Humans; MicroRNAs - genetics; Protein Folding; RNA Processing, Post-Transcriptional - genetics; RNA Splicing - genetics; RNA, Messenger - genetics; Signal Transduction - genetics; Transcription, Genetic - genetics; Unfolded Protein Response - genetics; X-Box Binding Protein 1 - genetics; ER stress; miRNA; XBP1; UPR
• ISSN: 1530-6860
• DOI: 10.1096/fj.201900600RR

During endoplasmic reticulum (ER) stress conditions, an adaptive signaling network termed the unfolded protein response (UPR) is activated. The UPR's function is to increase ER protein-folding capacity in order to attenuate ER stress, restore ER homeostasis, and, most importantly, promote cell survival. X-box-binding protein 1 (XBP1) is one component of the UPR and is a proadaptive transcription factor that is subject to transcriptional, post-transcriptional, and post-translational control. In the present study, we identified a post-transcriptional mechanism mediated by that governs the expression of both the spliced (active) and unspliced (latent) forms of mRNAs. We showed that directly attenuates spliced XBP1 ( ) mRNA levels during ER stress and thus regulates the proadaptive component of the UPR that is mediated by XBP1s without interfering with the induction of apoptotic responses.-Bartoszewska, S., Cabaj, A., Dąbrowski, M., Collawn, J. F., Bartoszewski, R. modulates X-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response.