RGDS- and SIKVAVS-Modified Superporous Poly(2-hydroxyethyl methacrylate) Scaffolds for Tissue Engineering Applications

• Published in: Macromolecular bioscience Vol. 16; no. 11; pp. 1621 - 1631
• Main Authors: Macková, Hana, Plichta, Zdeněk, Proks, Vladimír, Kotelnikov, Ilya, Kučka, Jan, Hlídková, Helena, Horák, Daniel, Kubinová, Šárka, Jiráková, Klára
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.11.2016; Wiley Subscription Services, Inc
• Subjects: Ammonium; Cell Line; Crystals; Humans; IKVAV; Mesenchymal Stromal Cells - cytology; Methylmethacrylates - chemistry; Methylmethacrylates - pharmacology; Neural Stem Cells - cytology; Neural Stem Cells - metabolism; Oligopeptides - chemistry; Oligopeptides - pharmacology; peptide; poly(2-hydroxyethyl methacrylate); RGD; Skin & tissue grafts; stem cell; Stem cells; Tissue engineering; Tissue Engineering - methods; Tissue Scaffolds - chemistry; IKVAV; peptide; stem cell; RGD; poly(2-hydroxyethyl methacrylate)
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201600159

Three‐dimensional hydrogel supports for mesenchymal and neural stem cells (NSCs) are promising materials for tissue engineering applications such as spinal cord repair. This study involves the preparation and characterization of superporous scaffolds based on a copolymer of 2‐hydroxyethyl and 2‐aminoethyl methacrylate (HEMA and AEMA) crosslinked with ethylene dimethacrylate. Ammonium oxalate is chosen as a suitable porogen because it consists of needle‐like crystals, allowing their parallel arrangement in the polymerization mold. The amino group of AEMA is used to immobilize RGDS and SIKVAVS peptide sequences with an N‐γ‐maleimidobutyryloxy succinimide ester linker. The amount of the peptide on the scaffold is determined using 125I radiolabeled SIKVAVS. Both RGDS‐ and SIKVAVS‐modified poly(2‐hydroxyethyl methacrylate) scaffolds serve as supports for culturing human mesenchymal stem cells (MSCs) and human fetal NSCs. The RGDS sequence is found to be better for MSC and NSC proliferation and growth than SIKVAVS. Superporous RGDS‐poly(2‐hydroxyethyl methacrylate) scaffold supports mesenchymal and neural stem cell attachment, proliferation, and growth. This makes the scaffold attractive for accommodation of cells in tissue engineering applications such as spinal cord repair.