Gene delivery to the airway

This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also de...

Full description

Saved in:
Bibliographic Details
Published inCurrent protocols in human genetics Vol. Chapter 13; p. Unit 13.9
Main Authors Keiser, Nicholas W, Engelhardt, John F
Format Journal Article
LanguageEnglish
Published United States 01.07.2013
Subjects
Animals
Cell Culture Techniques
Cell Line, Transformed
Epithelial Cells - metabolism
Gene Expression
Gene Transfer Techniques
Heterografts
Humans
Models, Animal
Respiratory Mucosa - metabolism
Transduction, Genetic - methods
Transgenes
Online AccessGet more information

Cover

More Information
Summary:This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of tracheal xenografts, as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.
ISSN:1934-8258
DOI:10.1002/0471142905.hg1309s78