In vitro studies on hormone-stimulated lipid mobilization from fat body and interconversion of haemolymph lipoproteins of Locusta migratoria

• Published in: Journal of insect physiology Vol. 30; no. 8; pp. 685,691 - 689,693
• Main Authors: Van Heusden, M.C., Van Der Horst, D.J., Beenakkers, A.M.Th
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 1984; Elsevier
• Subjects: Acrididae; adipokinetic hormone; animal physiology; arthropod pests; arthropods; Biochemistry. Physiology. Immunology; Biological and medical sciences; entomology; Fundamental and applied biological sciences. Psychology; haemolymph lipoproteins; In vitro; Insecta; Invertebrates; lipid mobilization; Locusta migratoria; octopamine; Physiology. Development; plant health; plant pests; octopamine; haemolymph lipoproteins; Locusta migratoria; lipid mobilization; In vitro; adipokinetic hormone; Adipose tissue; Insecta; Orthoptera; Hemolymph; Lipids; Chemical mediator; Lipoprotein; Metabolism; Acrididae; Arthropoda; Octopamine; Invertebrata; Adipokinetic hormone
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/0022-1910(84)90054-4

Both adipokinetic hormone and octopamine have a stimulating effect on lipid release from locust fat body in vitro, when incubated in diluted haemolymph. The presence of adipokinetic hormone results in the formation of the flight-specific haemolymph lipoprotein A + accepting the increased amount of lipids released into the incubation medium. In contrast, interconversions of lipoproteins do not occur when octopamine is added to the incubation medium, which is in line with the expectations: the lipid-mobilizing effect of octopamine is a limited and short-term effect. When fat body tissue is incubated with isolated haemolymph protein fractions, the lipid-mobilizing effect of adipokinetic hormone only occurs when the incubation medium contains both lipoprotein, A y and protein fraction C, resulting in the formation of lipoprotein A +. In similar control incubations with the hormone omitted, some lipoprotein A + is also formed (concomitant with a slight amount of lipid released), though significantly less than in incubations with hormone. Besides a stimulating function on lipolytic processes in the fat body, adipokinetic hormone is suggested to influence haemolymph lipoprotein rearrangement. A possible counteracting function of another factor in the haemolymph is discussed.