Effects of l‐arginine on the kidney levels of malondialdehyde in rats submitted to renal ischaemia‐reperfusion

• Published in: BJU international Vol. 88; no. 3; pp. 273 - 277
• Main Authors: Rhoden, E.L., Lucas, M.L., Pereira‐Lima, L., Rhoden, C.R., Souto, C.A.V.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.08.2001; Blackwell
• Subjects: Animals; Arginine - pharmacology; Biological and medical sciences; Creatinine - blood; ischaemia; kidney; Kidney - blood supply; Kidney - metabolism; l‐arginine; malondialdehyde; Malondialdehyde - metabolism; Medical sciences; Nephrology. Urinary tract diseases; nitric oxide; Nitric Oxide - blood; Rats; Rats, Wistar; Reperfusion; Reperfusion Injury - metabolism; Urinary system involvement in other diseases. Miscellaneous; Urinary tract. Prostate gland; Kidney disease; Urinary system disease; Pathophysiology; Rat; Rodentia; Cardiovascular disease; Exploration; Kidney; Vascular disease; Vertebrata; Mammalia; Reperfusion; Arginine; Ischemia; Aminoacid; Animal; Nitric oxide
• ISSN: 1464-4096; 1464-410X
• DOI: 10.1046/j.1464-410x.2001.02303.x

Objective To evaluate the effects of l‐arginine, a nitric oxide donor, on kidney levels of malondialdehyde (MDA, a product of cellular lipid peroxidation), serum creatinine levels, and urinary volume in rats undergoing unilateral renal ischaemia‐reperfusion. Materials and methods Wistar rats (117) were randomly distributed into three experimental groups (of four subgroups each) in which were assessed renal cell‐lipid peroxidation (kidney levels of MDA), serum creatinine levels and urinary volume. The rats underwent unilateral nephrectomy followed by contralateral renal ischaemia‐reperfusion with or with no pretreatment with l‐arginine (200 mg/kg) given intraperitoneally. Results Pretreatment with l‐arginine caused significantly higher kidney levels of MDA than in the untreated group (P < 0.05). Furthermore, l‐arginine given before surgery attenuated the increase in serum creatinine and significantly increased urinary volume in rats subjected to renal ischaemia‐reperfusion (P < 0.05). Conclusion l‐arginine tended to be of benefit for renal function during renal ischaemia‐reperfusion in rats. Pretreatment with l‐arginine (200 mg/kg intraperitoneally) seems to increase the renal damage by increasing kidney levels of MDA.