Biodegradation of erythromycin by Delftia lacustris RJJ‐61 and characterization of its erythromycin esterase

• Published in: Journal of basic microbiology Vol. 61; no. 1; pp. 55 - 62
• Main Authors: Ren, Jianjun, Wang, Zhenzhu, Deng, Liujie, Niu, Dongze, Fan, Bo, Huhe, Taoli, Li, Zhenzhen, Zhang, Jin, Li, Chunyu
• Format: Journal Article
• Language: English
• Published: Germany 01.01.2021
• Subjects: bacteria; biodegradation; Delftia; Delftia lacustris; environment; erythromycin; erythromycin esterase; Escherichia coli; esterases; genes; human health; molecular weight; mycelium; pollutants; biodegradation; erythromycin esterase; Delftia lacustris; erythromycin
• ISSN: 0233-111X; 1521-4028; 1521-4028
• DOI: 10.1002/jobm.202000613

The residual erythromycin in fermentation waste can pollute the environment and threaten human health. However, there are no effective approaches to remedy this issue. In this study, an erythromycin‐degrading bacterium named RJJ‐61 was isolated and identified as a strain of Delftia lacustris based on morphological and phylogenetic analyses. The degradation ability of this strain was also evaluated; it could degrade 45.18% of erythromycin at 35°C in 120 h. Furthermore, the key degradation gene ereA was cloned from strain RJJ‐61 and expressed in Escherichia coli BL21; the molecular weight of the expressed protein was ~45 kDa. The enzyme activity of EreA was 108.0 mU ml−1 at 35°C and pH 7.0. Finally, the EreA protein was used to degrade erythromycin from mycelial dregs and 50% diluted solution, and the removal rates in them were 41.42% and 69.78%, respectively. In summary, D. lacustris RJJ‐61 is a novel erythromycin‐degrading strain that has great potential to remove erythromycin pollutants from the environment.