Trends in proteomic analysis of human vitreous humor samples
Proteomic analysis of human vitreous humor (VH) may elucidate the pathogenesis of retinal ocular diseases and may provide information for the development of potential therapeutic targets due to its pivotal location near lens and retina. The discovery of whole VH proteome involves a complex analysis...
Saved in:
Published in | Electrophoresis Vol. 35; no. 17; pp. 2495 - 2508 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Germany
Blackwell Publishing Ltd
01.09.2014
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Proteomic analysis of human vitreous humor (VH) may elucidate the pathogenesis of retinal ocular diseases and may provide information for the development of potential therapeutic targets due to its pivotal location near lens and retina. The discovery of whole VH proteome involves a complex analysis of thousands of proteins simultaneously. Therefore, in proteomic studies the protein fractionation is important for reducing sample complexity, facilitating the access to the low‐abundant proteins, and recognizing them as biotargets for clinical research. Although several separation methods have been used, gel‐based proteomics are the most popular and versatile ones applied for global protein separation. However, chromatographic methods and its combination with other separation techniques are now beginning to be used as promising set‐ups for VH protein identification. This review attempts to offer an overview of the techniques currently used with VH, exploring its methodological demands, exposing its advantages, and helping the reader to plan future experiences. Moreover, this review shows the relevance of VH proteomic analysis as a tool for the study of the mechanisms underlying some ocular diseases and for the development of new therapeutic approaches. |
---|---|
Bibliography: | ICI and Santander Totta - No. CENTRO-07-ST24-FEDER-002014; No. CENTRO-07-ST24-FEDER-002015 University of Beira Interior - Health Sciences Research Centre (CICS) Bruno Bacher - No. Pest-C/SAU/UI0709/2011 istex:129B9BD660C8D5E4E25E523E85036792938E9F77 ark:/67375/WNG-Q5V6JMJZ-0 ArticleID:ELPS5152 These authors have contributed equally to this work. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.201400049 |