Interactions of a Periplasmic Binding Protein with a Tetradentate Siderophore Mimic

• Published in: Angewandte Chemie International Edition Vol. 52; no. 17; pp. 4595 - 4598
• Main Authors: Raines, Daniel J., Moroz, Olga V., Wilson, Keith S., Duhme-Klair, Anne-K.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 22.04.2013; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Amino Acid Sequence; Amino acids; Binding; Biomimetic Materials - chemistry; Coordination Complexes - chemistry; Electrostatics; iron uptake; metalloproteins; Models, Molecular; periplasmic binding proteins; Periplasmic Binding Proteins - chemistry; Pocket; protein structures; Proteins; Siderophores - chemistry; tetradentate siderophores
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.201300751

Iron‐bound structure: The ferric complex of a tetradentate siderophore mimic was synthesized and co‐crystallized with the periplasmic binding protein CeuE of Campylobacter jejuni. In addition to electrostatic and hydrogen‐bonding interactions between the binding pocket and the substrate, the structure showed direct coordination of two amino acid side chains to the FeIII center (orange, see figure).