Reduction of aflatoxin and fumonisin contamination in yellow corn by high-speed dual-wavelength sorting

• Published in: Cereal chemistry Vol. 81; no. 4; pp. 490 - 498
• Main Authors: Pearson, T.C, Wicklow, D.T, Pasikatan, M.C
• Format: Journal Article
• Language: English
• Published: St. Paul, MN The American Association of Cereal Chemists, Inc 01.07.2004; American Association of Cereal Chemists
• Subjects: aflatoxins; Aspergillus flavus; Biological and medical sciences; Cereal and baking product industries; corn; decontamination; food contamination; Food industries; food processing; food processing equipment; fumonisins; Fundamental and applied biological sciences. Psychology; fungal diseases of plants; Fusarium; high-speed dual-wavelength sorting; reflectance; sorting; spectrometers; yellow corn; Reduction; Yellow; High speed; Corn; Cereal; Fumonisin; Contamination; Sorting
• ISSN: 0009-0352; 1943-3638
• DOI: 10.1094/CCHEM.2004.81.4.490

A high-speed dual-wavelength sorter was tested for removing corn contaminated in the field with aflatoxin and fumonisin. To achieve accurate sorting, single kernel reflectance spectra (500-1,700 nm) were analyzed to select the optimal pair of optical filters to detect mycotoxin-contaminated corn during high-speed sorting. A routine, based on discriminant analysis, was developed to select the two absorbance bands in the spectra that would give the greatest classification accuracy. In a laboratory setting, and with the kernels stationary, absorbances at 750 and 1,200 nm could correctly identify >99% of the kernels as aflatoxin-contaminated (>100 ppb) or uncontaminated. A high-speed sorter was tested using the selected filter pair for corn samples inoculated with Aspergillus flavus; naturally infested corn grown in central Illinois; and naturally infested, commercially grown and harvested corn from eastern Kansas (2002 harvest). For the Kansas corn, the sorter was able to reduce aflatoxin levels by 81% from an initial average of 53 ppb, while fumonisin levels in the same grain samples were reduced an average of 85% from an initial level of 17 ppm. Similar reductions in mycotoxin levels were observed after high-speed sorting of A. flavus inoculated and naturally mold-infested corn grown in Illinois.