Tissue Impression Smears as a Supplementary Diagnostic Method for Histopathology in Cutaneous Leishmaniasis in Sri Lanka

Cutaneous leishmaniasis (CL) is diagnosed mainly by light microscopy of smears made using lesion material. Histopathology is usually done in atypical presentations or when lesion smears are negative. Tissue impression smears (TIS) made from skin biopsy specimens were compared with histopathology for...

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Published inThe American journal of tropical medicine and hygiene Vol. 98; no. 3; pp. 759 - 762
Main Authors Manamperi, Nuwani H, Chandu de Silva, M V, Pathirana, Nishantha, Abeyewickreme, Wimal, Karunaweera, Nadira D
Format Journal Article
LanguageEnglish
Published United States Institute of Tropical Medicine 01.01.2018
The American Society of Tropical Medicine and Hygiene
Subjects
Biopsy
Histocytochemistry - methods
Histopathology
Humans
Leishmania donovani - pathogenicity
Leishmania donovani - ultrastructure
Leishmaniasis, Cutaneous - diagnosis
Leishmaniasis, Cutaneous - parasitology
Leishmaniasis, Cutaneous - pathology
Microscopy
Parasitic diseases
Skin - parasitology
Skin - pathology
Sri Lanka
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Summary:Cutaneous leishmaniasis (CL) is diagnosed mainly by light microscopy of smears made using lesion material. Histopathology is usually done in atypical presentations or when lesion smears are negative. Tissue impression smears (TIS) made from skin biopsy specimens were compared with histopathology for the diagnosis of CL. Out of the 111 patients included, 83 (74.8%) were positive by either methods. The TIS was positive in 70.3% whereas histopathology was positive in 56.8% of patients. Tissue impression smears can be used as a supplementary diagnostic test that gives sensitive and rapid results when tissue biopsies are used as the source of lesion material for diagnosis of CL.
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The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or any other funding agency.
Financial support: Research reported in this publication was supported by the National Institute of Allergy And Infectious Diseases of the National Institutes of Health under Award Numbers R01AI099602 and U01AI136033, the University Grants Commission of Sri Lanka (research grant: UGC/VC/DRIC/PG/2013/KLN/03), and University of Kelaniya, Sri Lanka (research grant: RP/03/04/06/01/2014).
Authors’ addresses: Nuwani H. Manamperi, Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka, E-mail: nuwani_harsh@kln.ac.lk. M.V. Chandu de Silva, Department of Pathology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka, E-mail: desilva_vipula@yahoo.com. Nishantha Pathirana, Department of Dermatology, Army Hospital, Colombo, Sri Lanka, E-mail: kpnpathirana@yahoo.com. Wimal Abeyewickreme, Department of Parasitology, Faculty of Medicine, General Sir John Kotelawala Defence University, Rathmalana, Sri Lanka, E-mail: wabeyewickreme@yahoo.com. Nadira D. Karunaweera, Department of Parasitology, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka, E-mail: nadira@parasit.cmb.ac.lk.
ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.17-0748