Enhance wastewater biological treatment through the bacteria induced graphene oxide hydrogel

• Published in: Chemosphere (Oxford) Vol. 190; pp. 201 - 210
• Main Authors: Shen, Liang, Jin, Ziheng, Wang, Dian, Wang, Yuanpeng, Lu, Yinghua
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2018
• Subjects: Assembly; Bacteria; Bacteria - drug effects; Bacteria - metabolism; Biodegradation, Environmental; Biological wastewater treatment; Graphene; Graphite - chemistry; Graphite - metabolism; Hydrogel; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry; Hydrogel, Polyethylene Glycol Dimethacrylate - metabolism; Hydrogel, Polyethylene Glycol Dimethacrylate - pharmacology; Waste Water - microbiology; Water Purification - methods; Bacteria; Hydrogel; Biological wastewater treatment; Graphene; Assembly
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2017.09.105

The interaction between bacteria and graphene-family materials like pristine graphene, graphene oxide (GO) and reduced graphene oxide (rGO) is such an elusive issue that its implication in environmental biotechnology is unclear. Herein, two kinds of self-assembled bio-rGO-hydrogels (BGHs) were prepared by cultivating specific Shewanella sp. strains with GO solution for the first time. The microscopic examination by SEM, TEM and CLSM indicated a porous 3D structure of BGHs, in which live bacteria firmly anchored and extracellular polymeric substances (EPS) abundantly distributed. Spectra of XRD, FTIR, XPS and Raman further proved that GO was reduced to rGO by bacteria along with the gelation process, which suggests a potential green technique to produce graphene. Based on the characterization results, four mechanisms for the BGH formation were proposed, i.e., stacking, bridging, rolling and cross-linking of rGO sheets, through the synergistic effect of activities and EPS from special bacteria. More importantly, the BGHs obtained in this study were found able to achieve unique cleanup performance that the counterpart free bacteria could not fulfill, as exemplified in Congo red decolorization and Cr(VI) bioreduction. These findings therefore enlighten a prospective application of graphene materials for the biological treatment of wastewaters in the future. [Display omitted] •Some special bacteria can induce GO reduction and bio-rGO-hydrogel (BGH) formation.•BGH was characterized as 3D porous structure containing live bacteria, EPS and rGO.•Assemble mechanisms were proposed as stacking, bridging, rolling and cross-linking.•BGH was proven superior to free bacteria when removing dye and Cr(VI).•BGH suggests a new use of graphene material for biological wastewater treatment.