Study of morphine-induced dependence in gonadectomized male and female mice

• Published in: Pharmacology, biochemistry and behavior Vol. 91; no. 4; pp. 604 - 609
• Main Authors: Sadeghi, Mahsa, Sianati, Setareh, Anaraki, Dina Kalbasi, Ghasemi, Mehdi, Paydar, Mehrak Javadi, Sharif, Behrang, Mehr, Shahram Ejtemaei, Dehpour, Ahmad Reza
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Inc 01.02.2009; Elsevier
• Subjects: Animals; Biological and medical sciences; Estrogen; Estrogens - pharmacology; Female; Male; Medical sciences; Mice; Morphine; Morphine dependence; Morphine Dependence - psychology; Naloxone - pharmacology; Narcotic Antagonists - pharmacology; Orchiectomy; Ovariectomy; Progesterone; Progesterone - pharmacology; Sex Characteristics; Sex difference; Substance Withdrawal Syndrome - psychology; Testosterone; Testosterone - pharmacology; Weight Loss - drug effects; Testosterone; Morphine dependence; Estrogen; Morphine; Mice; Progesterone; Sex difference; Androgen; Rodentia; Sex; Opiates; Male; Narcotic analgesic; Ovarian hormone; Progestagen; Vertebrata; Mammalia; Mouse; Animal; Dependence; Female; Drug of abuse; Testicular hormone; Sex steroid hormone
• ISSN: 0091-3057; 1873-5177
• DOI: 10.1016/j.pbb.2008.09.015

In this study we evaluated the effects of sex difference and also sex hormones on the naloxone-precipitated morphine withdrawal in both orchidectomized (ORC) male and ovariectomized (OVX) female mice. Morphine (50, 50 and 75 mg/kg/day for 4 days, s.c.) was administered to animals and at 5th day naloxone (4 mg/kg, i.p.)-precipitated morphine withdrawal signs, jumpings and the percentage of weight loss, were measured. There was no significant alteration in withdrawal jumpings between male and female mice, though weight loss was significantly higher in male ones. Jumpings was significantly lower in both OVX and ORC mice and percentage of weight loss was significantly higher in OVX mice than corresponding non-operated or sham animals. In OVX mice, E 2V (10 mg/kg, s.c.) increased number of jumpings and decreased percentage of weight loss. Progesterone (25 mg/kg, s.c.) had no effect on jumpings, whereas it decreased weight loss in OVX mice. Testosterone (2.5 mg/kg, s.c.) increased jumpings in ORC mice while it had no effect on percentage of weight loss. Our results demonstrated that sex hormones could play a role in the morphine withdrawal syndrome in both ORC male and OVX female mice.