Direct evidence for Mendelian inheritance of the variations in the ribosomal protein gene introns in yellowfin tuna (Thunnus albacares)

• Published in: Marine biotechnology (New York, N.Y.) Vol. 3; no. 1; pp. 22 - 26
• Main Authors: Chow, S, Scholey, V P, Nakazawa, A, Margulies, D, Wexler, J B, Olson, R J, Hazama, K
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.01.2001
• Subjects: Feasibility studies; Fish populations; Gene polymorphism; Genotypes; Heredity; Heterozygosity; Introns; Larvae; Marine; Marine fishes; Nucleotide sequence; Offspring; Parents; PCR; Pedigree; Polymerase chain reaction; Polymorphism; Population genetics; Population studies; Proteins; Restriction fragment length polymorphism; ribosomal protein S7; Thunnus albacares; Tuna
• ISSN: 1436-2228; 1436-2236
• DOI: 10.1007/s101260000031

Restriction fragment length polymorphism found in the S7 ribosomal protein gene introns of yellowfin tuna (Thunnus albacares) was compared between a single pair of parents and their offspring. The sizes of the first intron ( RP1) and second intron ( RP2) amplified by polymerase chain reaction were 810 bp and 1400 bp, respectively. The dam and sire had different restriction types from one another in HhaI and RsaI digestions for RP1 and in DdeI, HhaI, and ScrFI digestions for RP2. Putative genotypes in both introns of 64 larvae were found to be segregated in Mendelian proportions. Genotype distributions in a wild yellowfin tuna sample ( n = 34) were in Hardy-Weinberg proportions, and observed heterozygosity ranged from 0.149 to 0.388. This study presents novel Mendelian markers, which are feasible for tuna population genetic study and pedigree analysis.