Homeodomain binding sites in the 5′ flanking region of the Bombyx mori silk fibroin light-chain gene

• Published in: Journal of molecular biology Vol. 213; no. 3; pp. 395 - 398
• Main Authors: Hui, Chi-chung, Suzuki, Yoshiaki, Kikuchi, Yoshimi, Mizuno, Shigeki
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 05.06.1990; Elsevier
• Subjects: Amino Acid Sequence; Animals; Bacterial Proteins; Base Composition; Base Sequence; Binding Sites; Biological and medical sciences; Bombyx - genetics; Bombyx mori; Cloning, Molecular; DNA - analysis; DNA-Binding Proteins - genetics; Drosophila - genetics; Drosophila Proteins; Escherichia coli - genetics; Escherichia coli Proteins; Fibroins - genetics; Fundamental and applied biological sciences. Psychology; Gene expression; genes; Genes, Homeobox; genetic stability; Homeodomain Proteins; homeotic genes; Insect Proteins; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Promoter Regions, Genetic; proteins; Proteins - genetics; Restriction Mapping; Silk; transcription (genetics); Transcription Factors; Gene product; Transcription promoter; Insecta; Drosophila; Molecular interaction; Bombyx mori; Binding site; Drosophilidae; Proteins; Regulation(control); Silk; Gene; Arthropoda; Bombycidae; Lepidoptera; Fibroin; Invertebrata; Diptera
• ISSN: 0022-2836; 1089-8638
• DOI: 10.1016/S0022-2836(05)80201-3

Multiple A + T-rich stretches in the 5′ flanking region of the Bombyx mori fibroin light-chain gene have been shown to bind two Drosophila homeodomain proteins, EVE ( even-skipped) and ZEN ( zerknüllt), with high affinities. Some of these sites fall into a class that has the established consensus sequence of the binding sites (TCAATTAAAT) for a diverse group of Drosophila homeodomain proteins, while others are quite heterogeneous except that they all possess a core TAAT motif. Since clusters of homeodomain binding sites can also be found in the promoters of other silk protein genes, the fibroin gene and the sericin-1 gene, these observations suggest a possible involvement of some homeobox genes in the regulation of a group of silk protein genes.