Photodynamic effects of zinc oxide nanowires in skin cancer and fibroblast

• Published in: Lasers in medical science Vol. 29; no. 3; pp. 1189 - 1194
• Main Authors: Fakhar-e-Alam, Muhammad, Kishwar, S., Willander, M.
• Format: Journal Article
• Language: English
• Published: London Springer London 01.05.2014; Springer Nature B.V
• Subjects: Cell Line, Tumor; Cell Survival - drug effects; Cell Survival - radiation effects; Dentistry; Drug Delivery Systems; Fibroblasts - drug effects; Fibroblasts - radiation effects; Humans; Lasers; Male; Medicine; Medicine & Public Health; Melanoma - drug therapy; Nanowires; Nanowires - therapeutic use; Nanowires - ultrastructure; Optical Devices; Optics; Original Article; Photochemotherapy; Photodynamic therapy; Photonics; Photosensitizing Agents - therapeutic use; Protoporphyrins - therapeutic use; Quantum Optics; Skin cancer; Skin Neoplasms - drug therapy; TECHNOLOGY; TEKNIKVETENSKAP; Ultraviolet Rays; Zinc Oxide - therapeutic use; Zinc oxides; Protoporphyrin 1X (PpIX); ZnO nanowires (ZnO NWs)
• ISSN: 0268-8921; 1435-604X; 1435-604X
• DOI: 10.1007/s10103-013-1501-4

Cytotoxic effects of zinc oxide (ZnO) nanomaterials, individual and conjugated with a photosensitizer (protoporphyrin IX), were studied in the presence and absence of ultraviolet light exposure (240 nm of light wavelength for a very short time exposure) in cell cultures of human normal and cancerous skin models. Zinc Oxide nanowires (ZnO NWs) were grown on the capillary tip and conjugated with protoporphyrin IX (PpIX). This coated tip was used as tool/pointer for intracellular drug delivery protocol in suggested normal as well as carcinogenic cellular models. After true delivery of optimal drug, the labelled biological model was irradiated with UV-A, which led to a loss of mitochondrial membrane potential, as tested by neutral red assay (NRA).