Single-molecule optofluidic microsensor with interface whispering gallery modes

Label-free sensors are highly desirable for biological analysis and early-stage disease diagnosis. Optical evanescent sensors have shown extraordinary ability in label-free detection, but their potentials have not been fully exploited because of the weak evanescent field tails at the sensing surface...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 119; no. 6; pp. 1 - 6
Main Authors Yu, Xiao-Chong, Tang, Shui-Jing, Liu, Wenjing, Xu, Yinglun, Gong, Qihuang, Chen, You-Ling, Xiao, Yun-Feng
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences 08.02.2022
Subjects
Biological analysis
Biological Sciences
Biosensing Techniques - methods
Biosensors
Microfluidics
Microfluidics - methods
Molecular weight
Nanotechnology - methods
Physical Sciences
Whispering gallery modes
whispering gallery microresonator
interfacial molecular detection
optofluidic biosensor
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ISSN0027-8424
1091-6490
1091-6490
DOI10.1073/pnas.2108678119

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Summary:Label-free sensors are highly desirable for biological analysis and early-stage disease diagnosis. Optical evanescent sensors have shown extraordinary ability in label-free detection, but their potentials have not been fully exploited because of the weak evanescent field tails at the sensing surfaces. Here, we report an ultrasensitive optofluidic biosensor with interface whispering gallery modes in a microbubble cavity. The interface modes feature both the peak of electromagnetic-field intensity at the sensing surface and high-Q factors even in a small-sized cavity, enabling a detection limit as low as 0.3 pg/cm². The sample consumption can be pushed down to 10 pL due to the intrinsically integrated microfluidic channel. Furthermore, detection of single DNA with 8 kDa molecular weight is realized by the plasmonic-enhanced interface mode.
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Author contributions: X.-C.Y., S.-J.T., and Y.X. performed the experiments; X.-C.Y. carried out the simulation and numerical calculation; X.-C.Y., S.-J.T., W.L., Q.G., and Y.-L.C. analyzed the data; X.-C.Y., S.-J.T., W.L., Y.X., Q.G., Y.-L.C., and Y.-F.X. contributed to the discussions and wrote the manuscript; S.-J.T. and Y.-F.X. conceived the idea; and Y.-L.C. and Y.-F.X. supervised the project.
1X.-C.Y. and S.-J.T. contributed equally to this work.
Edited by Douglas Shepherd, Arizona State University, Tempe, AZ; received May 13, 2021; accepted December 23, 2021 by Editorial Board Member John W. Sedat
ISSN:0027-8424
1091-6490
1091-6490
DOI:10.1073/pnas.2108678119